核苷磷酸化酶

目的分别构建三种启动子调控的大肠杆菌嘌呤核苷磷酸化酶（PNP）基因表达载体，检测、分析三者的表达差异。

Objective To construct three expression vectors harboring PNP ( purine nucleoside phosphorylase ) gene under three different promoters .

胸苷磷酸化酶（thymidinephosphorylase.TP）是嘧啶核苷磷酸化酶家族成员之一，是合成、限制细胞生长的必需酶。

Thymidine phosphorylase is one of the pyrimidine nucleoside phosphorylase family , it is essential for synthesis and restriction of cell growth .

目的：探讨卵巢肿瘤中血管内皮生长因子（VEGF）、vEGFmRNA、胸腺嘧啶脱氧核苷磷酸化酶（TP）和TPmRNA的表达及其临床意义。

Objectives : To study the expression of VEGF , TP and their mRNA in ovarian tumors as well as their clinical significance .

方法：采用RT-PCR法检测了28例胃癌、14例肺癌和22例结肠癌组织及其远癌正常组织中尿嘧啶核苷磷酸化酶（UP）基因的表达。

Methods : The mRNA level of UP gene was examined using RT-PCR in 28 cases of stomach carcinoma , 14 lung cancer and 22 colon carcinoma .

目的：研究卵巢肿瘤中胸腺嘧啶脱氧核苷磷酸化酶（TP）及其mRNA的表达与微血管密度（MVD）的关系及其临床意义。

Objective : To study the relationship between expressions of TP , TP mRNA and microvessel density ( MVD ) and their clinical significance in ovarian tumors .

目的通过检测乳腺癌标本中胸苷酸合成酶（TS）、胸腺嘧啶脱氧核苷磷酸化酶（TP）、二氟嘧啶脱氢酶（DPD）表达情况，探讨乳腺癌的生物学行为与上述3个指标之间的相关性。

Objective To investigate the relationships among the expression of thymidylate synthase ( TS ), thymidine phosphorylase ( TP ), and dihydropyrimidine dehydrogenase ( DPD ) and the prognosis of breast cancer .

胸苷酸合成酶、胸腺嘧啶脱氧核苷磷酸化酶及二氟嘧啶脱氢酶的表达与乳腺癌预后的相关性目的：检测北欧和中国人乳癌患者TP、TS、DPDmRNA水平的差异。

Aim : To investigate the difference between the mRNA levels of thymidine phosphorylase ( TP ), thymidylate synthase ( TS ), and dihydropyrimidine dehydrogenase ( DPD ) between Chinese and North European breast cancer patients .

目的：探讨胸腺嘧啶核苷磷酸化酶（TP）与子宫内膜癌的临床病理特征、无瘤生存率、微血管密度（MVD）的关系。

To study the relationships between the expression of thymidine phosphorylase ( TP ), the density of microvessels ( MVD ) of the tumor , the clinical and pathological features and tumor free survival rate in endometrial carcinoma .

目的：优化嘧啶核苷磷酸化酶（PyNPase）基因工程菌的发酵条件。

Objective : To obtained the optimized fermentation conditions of reconbinant pyrimidine nucleoside phosphorylase ( PyNPase ) .

嘌呤核苷磷酸化酶/氟达拉滨（PNP/fludarabine）自杀基因系统对人肝癌细胞系BEL7402体外杀伤效应的研究

Study on Killing Effects of Purine Nucleoside Phosphorylase / Fludarabine ( PNP / fludarabine ) Suicide Gene System on Human Hepatocellular Carcinoma Cell Line BEL7402 in vitro

产气肠杆菌突变株EAM-Z1的培养和核苷磷酸化酶活力的研究

Studies on the Culture Conditions of E. aerogenes Mutant EAM-Z 1 and its Phosphorylase Activity

目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。

Objective To study the effects E.coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect .

嘧啶核苷磷酸化酶（PyNPase）是嘧啶核苷补救代谢途径中的关键酶，广泛分布于微生物及动物组织细胞中。

Pyrimidine nucleoside phosphorylase ( PyNPase ), a key enzyme in the pyrimidine salvage pathway , is broadly distributed in various microorganism and mammalian cells .

以淋巴细胞减少症和发育缓迟为临床表现的嘌呤核苷磷酸化酶缺陷（PNPdef）1例：经脐带血移植得以成功治疗

Purine nucleoside phosphorylase deficiency ( PNPdef ) presenting with lymphopenia and developmental delay : Successful correction with umbilical cord blood transplantation

基于毛细管电泳在柱酶微反应器技术筛选嘌呤核苷磷酸化酶抑制剂

Purine nucleoside phosphorylase inhibitor screening using immobilized enzyme reactor by capillary electrophoresis

嘌呤核苷磷酸化酶基因的克隆及原核表达载体的构建

Cloning and expression of purine nucleoside phosphorylase in Escherichia coli

嘧啶核苷磷酸化酶与抗癌药物治疗效果的评价

Pyrimidine Nucleoside Phosphorylase and the Evaluation of Anticancer Drugs

γ线对人的红细胞嘌呤核苷磷酸化酶的影响

Effect of gamma irradiation on the activity of human erythrocyte purine nucleoside phosphorylase

γ射线对红细胞嘌呤核苷磷酸化酶的影响二、酶的某些性质

Effect of irradiation on erythrocyte purine nucleoside phosphorylase 2.some properties of the enzyme

胸腺嘧啶核苷磷酸化酶与子宫内膜癌关系的研究

A study on thymidine phosphorylase in endometrial carcinoma

生长温度对核苷磷酸化酶的影响

Effect of Growth Temperature on Nucleoside Phosphorylase

使用高效液相色谱技术测定嘌呤代谢产物（尿酸、黄嘌呤、次黄嘌呤、次黄苷）及嘌呤核苷磷酸化酶的活性。

The activity of purine nucleoside phosphorylase and release of purine were assayed by HPLC .

人结直肠癌组织中胸腺嘧啶核苷磷酸化酶和二氢嘧啶脱氢酶的活性变化及其意义

Clinicopathologic and prognostic significance of thymidine phosphorylase and dihydropyrimidine dehydrogenase activity in human colorectal carcinoma

合成利巴韦林的关键酶嘌呤核苷磷酸化酶的原核表达及活性分析

Prokaryotic Expression and Functional Analysis of Purine Nucleoside Phosphorylase of the Key Enzyme for Ribavirin Produced

嘧啶核苷磷酸化酶的α和α/β结构域刚性移动的动力学模拟

Dynamics Simulation of Rigid Body Movement of the α and α / β Domains of Pyrimidine Nucleoside Phosphorylase

离体与整体照射对大鼠红细胞嘌呤核苷磷酸化酶活力与红细胞膜脂质过氧化值的影响

Effect of gamma irradiation on erythrocyte purine nucleoside phosphorylase activity and erythrocyte membrane lipid peroxidation in vitro and in vivo

尿嘧啶核苷磷酸化酶基因在胃癌、肺癌和结肠癌组织中的表达水平及其临床意义

The differential expressions of uridine phosphorylase ( UPase ) gene in human gastric , colon , lung cancers and its clinical significance

目的：分析尿嘧啶核苷磷酸化酶基因表达水平与胃癌、肺癌和结肠癌的预后相关性。

Objective : To investigate the relationship between the expression levels of uridine phosphorylase ( UP ) gene and the prognosis of patients with the gastric , colon and lung carcinomas .

嘌呤核苷磷酸化酶是嘌呤核苷补救代谢途径中的关键酶，在抗肿瘤基因治疗、核苷类药物合成等方面具有广泛的应用。

Purine nucleoside phosphorylase ( PNPase , E.C.2.4.2.1 ) is a key enzyme in the purine salvage metabolic pathway . It could catalyze the synthesis of nucleosides , and has great potential in anti-tumour gene therapy .

溴氰菊酯对大鼠肝钙&腺嘌呤核苷三磷酸酶、磷酸化酶a的影响

Effect of decamethrin on the activities of Ca ~ [ 2 + ] - ATPase and phosphorylase a of rat liver