柚皮

HPLC法测定不同产地陈皮药材中橙皮苷的含量生产区对毛橘红果皮及花中柚皮苷含量的影响

Determination of Hesperidin in Pericarpium Citri Reticulatae from Different Producing area by HPLC

方法：HPLC测定黄酮类成分橙皮苷和柚皮苷含量、生物碱类辛弗林含量，药典法测定挥发油含量。

Method : HPLC analysis was performed to detect the contents of hesperidin , naringin and synephrine .

HPLC法测定血府逐瘀颗粒中芍药苷、阿魏酸、柚皮苷及甘草酸的含量

ü RP-HPLC analysis of paeoniflorin , ferulaic acid , naringin and glycyrrhizic acid in Xuefu Zhuyu granules

pH值和温度对柚皮提取物的抑菌活性均有影响，SPE在偏酸性条件下抑菌效果较好；

It is seen that pH value and temperature affect the antibacterial activity of the SPE .

柚皮素对人髓系白血病K562细胞增殖的作用

Effect and Mechanism of Naringenin on the Proliferation of Human Leukemia K562 Cell Line

同时采用RP-HPLC法测定柚皮苷含量。

The content of naringin was determined by reversed phase high-performance liquid chromatography ( RP-HPLC ) .

柚皮苷对大鼠BMSCs增殖及成软骨分化的影响

Effects of Naringin ( NG ) on the Proliferation and Differentiation into Chondrocytes of the Rat BMSCs

主要工作有：（1）以制备液相分离柚皮苷和新橙皮苷为研究对象，建立了色谱分离的平衡驱动模型（ED模型）。对于其中吸附等温线的测定，本文采用了由G。

First , an equilibrium-dispersive ( ED ) model was used to describe the chromatographic separation of Naringin and Neohesperidin .

对经AB-8大孔树脂纯化后的柚皮黄酮进行高效液相色谱（HPLC）分析发现，柚皮黄酮主要为黄酮甙类。

High performance liquid chromatography ( HPLC ) analysis result show that the obtained flavonoids is mainly flavone glycoside .

磷铝与硼铝互作对柚叶片和根系有机酸代谢的影响HPLC法测定不同规格酸橙枳实中新橙皮苷和柚皮苷的含量

Effects of Phosphorus-Aluminum and Boron-Aluminum Interactions on Organic Acid Metabolism in the Leaves and Roots of Pummelo HPLC determination of neohesperidin and naringin in Fructus Aurantii Immaturus of different specifications

目的：建立检测柑橘类黄酮（柚皮苷、橙皮苷、新橙皮苷、橙黄酮、川陈皮素）的中药材质量控制用快速HPLC方法。

AIM : To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids , naringin , hesperidin , neohesperidin , sinensetin and nobiletin .

目的：探讨柚皮素（naringenin）对人慢性髓系白血病K562细胞株生长的影响及其作用机制。

Objective : To investigate the effect and mechanism of naringenin on human leukemia K562 cell line .

将ED模型应用到柚皮苷和新橙皮苷色谱分离操作条件的优化。

Then , taken the yield as the objective function , the built ED model was used to determine the optimum separation conditions of Naringin and Neohesperidin .

结果：柚皮苷含量在0.132～0.660μg范围呈良好的线性关系，平均回收率为96.78%，RSD为1.3%。

Results : The content of shaddock ped glucoside on HPLC was linear in the range of 0.132-0.660 μ g. The average recovery was 96.78 % , RSD was 1.3 % .

流式细胞仪分析证实柚皮素能使K562细胞聚积在G0/G1期，S期细胞减少；

Observation by flow cytometry indicated that K562 cells were blocked in G0 / G1 phase of cell cycle in naringenin group , and S phase cells were remarkably decreased .

柚皮素和橙皮素对重组人肌醇磷脂3-激酶p110β催化亚基的影响

Effect of naringenin and hesperetin on recombinant human phosphoinositide 3-kinase p110 β catalytic subunit

柚皮素是一种广泛存在于植物和中药中的天然黄酮类化合物。有研究显示发现其具有多种药理活性，主要有抗氧化、抗衰老、抗肿瘤、抗动脉粥样硬化、DNA修复作用等。

Naringenin is a natural flavonoids , widely present in plants and Chinese traditional medicine , Studies show that variety of pharmacological activities have been found , such as the main antioxidant , anti-aging , anti-tumor , anti-atherosclerosis , the role of DNA repairment .

柚皮黄酮化合物提取物能显著降低蛋黄乳诱导高胆固醇血症小鼠血清的TC、TG和LDL-C的浓度，并提高HDL-C浓度（P<0.05）。

The pomelo flavonoids extracts can decrease TG , TC and LDL-C level but increase HDL-C level in mice with high blood lipids induced by egg yolk ( P < 0.05 ) .

现代中药药物化学研究证实骨碎补中的有效成分主要为一种黄酮类化合物&柚皮苷（naringin）。

By the way of medicinal chemistry , it has been proved that the virtual component of Gu-Sui-Bu is naringin , a type of flavone .

用乙醇作提取剂提取柚皮，将提取物经过初步分离纯化得到黄酮含量为109%的柚皮提取物（SPE），并对其抑制细菌和霉菌的作用进行了研究。

Shaddock peels extracted ( SPE ) with the content of flavone of 10.9 % was obtained using ethanol extraction and its antimicrobial activity against a series of bacterium and mold was studied .

结果表明，离子交换法提取柚皮果胶的最佳工艺条件是：732阳离子交换树脂用量7%，提取液酸度pH值2.0，提取温度85℃，提取时间2.5h，料液比1∶30。

As a result , the best technical condition of the pectin from pomelo peel with the method of ion-exchange was : 732 cation exchange resins , 7 % ; draw acidity , pH 2.0 ; draw temperature , 85 ℃;

【结果】化橘红中柚皮苷与野漆树苷在紫外光254nm下显示分开的暗斑点；

[ Results ] Spots of naringin and rhoifolin varied when the wavelength of ultraviolet was at 254 nm .

目的：研究柚皮素和橙皮素对重组人肌醇磷脂3-激酶（PI3-K）p110β催化亚基酶活性的影响。

Objective : To study the effect of naringenin and hesperetin on activity of recombinant human phosphoinositide 3-kinase ( PI3-K ) p110 β catalytic subunit .

结果表明：柚皮提取物的最佳提取条件是：料液比为7：3，柚皮原料在60℃的水浴锅中加热3h，在西式火腿中加入1.6%的该柚皮浸提液对亚硝胺的阻断效果最好。

The result shows the optimum condition for extraction of citrus grands peel is that the ratio of water and citrus grandis peel is 7:3 in the incubation at 60 ℃ for three hours , and the best adding content is 1.6 % .

结果表明：柚皮苷和新北美圣草苷分别在10～400mg·mL~（-1）和12～480mg·mL~（-1）范围内，线性良好（r≥0.9997）；

A good linearity between peak area and the concentration was found in the range of 10 ~ 400 g mL-1 and 12 ~ 480 g mL-1 for naringin and neoeriocitrin ( r 0.999 7 ), respectively .

方法室温条件下超声提取柚皮苷，HPLC法测定其含量，ODS色谱柱，以乙腈-8%冰醋酸（15∶85）为流动相，检测波长283nm。

Methods At room temperature and by using ultrasonic extraction , HPLC was performed to determine naringin content on ODS chromatographic column . The mobile phase consisted of a mixture of acetonitrile and 8 % acetic acid ( 15 ∶ 85 ) and detection wavelength was at 283 nm .

研究者还发现，柚皮素能拮抗药物摄取的关键转运体，OATP1A2，它能使药物从小肠转运到血液中。

The team also found that naringin appeared to block a key drug uptake transporter , OATP1A2 , which has a role in transporting drugs from the small intestine into the bloodstream .

他认为葡萄柚汁不是唯一影响药物吸收的果汁，因为橙汁和苹果汁中也含有柚皮素样物质，可能抑制OATP1A2。

He added that grapefruit juice may not be the only type of fruit juice that affects drug absorption , as orange and apple juices also contain naringin-like substances that may inhibit OATP1A2 .

柚皮色素中活性物质的分离及鉴定

Isolation and Structure Identification of Active Substance in Pomelo Peel Pigment

影响骨碎补浸膏中柚皮苷含量的因素研究

Influence of Different Extraction Conditions on Naringin Content in Drynaria Extract