杂交核酸

本文报道地里纤恙螨雄虫人工接种恙虫病立克次体后与雌虫配对培养和传代，应用幼虫叮咬小鼠分离立克次体和PCR结合核酸杂交（核酸杂交检测PCR产物）检测子代体内立克次体的结果。

This paper reports the results of the detection of Rickettsia tsutsugamushi in the offsprings of Leptotrombidium deliense which male parents were infected through inoculating by using PCR combine DNA-DNA hybridization technique and traditional technique .

核酸探针杂交是核酸研究中一项最基本的实验技术。

Hybridization with nucleic probe is one of the most basic technologies in the research of nucleic acid .

本文介绍了核酸探针和制备技术，标记技术、核酸分子杂交方法及核酸探针的应用状况。

The nucleic acid probe , manufacture and labelling technology , hybrid method of nucleic acid molecule , and the current application status of nucleic acid probe were introduced in this paper .

杂交瘤细胞核糖核酸抗病毒作用

Effect of anti-virus of RNA from hybridoma cell

方法：采用原位杂交和斑点杂交等核酸分子生物学技术，观测大鼠烧伤和烧冲复合伤后肝组织ET－1、ETR及肿瘤坏死因子（TNF－α）的转录表达变化及相互关系。

Methods : The changes of transcription and expression of ET 1 , ETR and TNF α in the liver after combined burn blast injury and the relationship among them were determined with in situ hybridization , dot hybridization and radioimmunoassay .

磁性纳米颗粒的应用可简便且有效地将杂交复合体与未杂交核酸或过量的检测探针分离，大大降低非特异核酸分子的干扰，提高了检测灵敏度；

Magnetic nanoparticles can separate the free nucleic acid or excessive probes from probes-target DNA complex easily and effectively , which improved the detection sensitivity .