无血清培养液

该细胞在添加胰岛素、氢化可的松以及羊催乳素的无血清培养液中诱导培养时，用RT-PCR方法检测到了β-酪蛋白基因的转录。

The transcription of the beta - casein gene was detected by RT - PCR , when the purified cells were induced with insulin , hydrocortisone and prolactin in the culture medium without serum .

用KC无血清培养液培养，光镜下观察HKC形态；

The HKCs were then cultured in KCS serum-free culture medium . The morphology of HKCs were observed under light microscope .

目的探讨无血清培养液培养人头皮毛乳头细胞（dermalpapillaecells，DPC）的可行性，并研究其生长特性。

Objective To explore the feasibility of culturing dermal papillae cells ( DPC ) of hu - man hair in a serum-flee medium , and to observe the growth characteristics of these cells .

分离新生Wistar鼠海马，采用添加B27的无血清培养液进行海马神经元原代培养，动态观察海马神经元形态学变化；

Hippocampal neurons of neonatal Wistar rats were isolated and cultured in the serum-free B27 supplemented culture media . The process of hippocampal neurons growth was observed .

方法小鼠腹腔内连续3d注射肌匀浆和淀粉混合物，然后分离小鼠腹腔巨噬细胞，在无血清培养液中培养48h后收集上清。

Methods After a 3-day intraperitoneal injection with a mixture of muscle homogenate and starch , macrophages were obtained from mouse peritoneal cavity and cultured for 48 hours in serum-free DMEM / F12.Then the cultured medium was harvested .

结果：换无血清培养液后，成纤维细胞迅速死亡，S100免疫荧光染色雪旺细胞和嗅鞘细胞的纯度可达98%～99%。

Results showed that fibroblast rapidly died in free-serum media , the purity of Schwann cells and olfactory ensheath cells were up to 98 % ~ 99 % using immunofluorescent staining .

方法取新生1周内乳鼠的大脑皮质，机械分离出神经干细胞，无血清培养液（DMEM/F12+bFGF+EGF+B27）培养，免疫组化SABC法对神经干细胞进行鉴定；

Methods NSCs harvested from the cerebral cortex of neonatal one-week mice were triturated and cultivated in serum-free DMEM / F12 + bFGF + EGF + B27 , then identified by the immunohistochemistry SABC method .

建立SD大鼠老龄骨质疏松骨折的动物模型，实验组将SD乳鼠颅骨体外培养的成骨细胞移植到SD雌性大鼠骨质疏松性骨折部位，对照组注入等量无血清培养液。

After fracture models of osteoporosis rats were established , in vitro cultured osteoblasts from the skull of SD neonatal rats were implanted into the fractured site of female SD rats in the experimental group , while the control group was injected with serum-free medium of the same volume .

高转移腺样囊性癌细胞系ACC-M无血清培养液中TGF-β1的分泌量亦低于任何其它细胞。

The level of TGF - β 1 secreted by ACC-M cells to the culture without serum was also lower than that of any of the other carcinoma cells or normal cells .

在培养液中添加不同浓度（10%、5%）的NBS及无血清培养液全程培养时，3组之间卵裂率（65.6%、60.0%、58.0%）差异不显著（P>0.05）；

10 % and 5 % NBS was added in the culture medium respectively and free NBS for full process culture . There were no significant differences among these three groups on cleavage rate ( 65.6 % , 60.0 % , 58.0 % ) .

大鼠α-谷氨酰转肽酶阳性肝细胞在无血清培养液中的生长

The growth of rat γ - glutamyltranspeptidase - positive hepatocytes in serum-free medium

结论：应用无血清培养液可进行气管黏膜纤毛上皮细胞的体外培养。组织块培养法培养的纤毛上皮细胞的分化能力保持较好。

Conclusion : Tracheal membrane epithelium cells can be cultured by serum free medium .

在无血清培养液中，一些生长因子和细胞因子的加入是必须的。

In serum free culture media , some growth factors and cytokines were essential .

空白对照组输注等量无血清培养液。

The mice in the blank control group were infused with isovolume serum-free culture medium .

利用无血清培养液培养小鼠胚胎脊髓、脊神经节的神经元，研究血清对髓鞘形成的影响。

Embryonic mouse spinal cord and dorsal root ganglion neurons were cultured in serum-free medium for4 weeks .

结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。

It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium .

结论应用组织块培养法结合无血清培养液可以成功建立原代人近端肾小管上皮细胞系。

Conclusion Primary tissue culture of proximal tubular epithelial cells combined with serum-free culture medium can be established in human .

方法取雌性新西兰大白兔阴道粘膜组织小块，胶原酶Ⅳ和胰蛋白酶联合消化分离法收集上皮细胞，接种于角朊细胞无血清培养液中静置培养、传代。

Methods Vaginal epithelial cell were harvested from the New Zealand White rabbit vaginal specimens by digestion with collagenase type ⅳ and trypsin .

结果：人牙周膜成纤维细胞在无血清培养液条件下可以生长和增殖，但与含血清培养液相比，其增殖速率变缓，分化明显。

RESULTS : Human periodontal ligament fibroblasts could growth and proliferate in serum-free medium , but in comparing with those in serum-containing medium , their proliferation rate decreased and their differentiation became earlier .

使用无血清培养液来进行卵母细胞的体外成熟培养和胚胎的发育培养，尽管近年来有人在不断尝试，但是还存在一定的要改进的地方。

The use of serum-free medium for oocyte maturation embryo culture in vitro and the development , although in recent years it was constantly trying , but there is still some places to improve .

结果在添加有纤维细胞生长因子的无血清培养液中，少量接种的单个细胞会增殖并形成悬浮于细胞培养液中的细胞克隆。

Results In the FGF added serum-free medium , neural cells experienced a large scale death within 48h after being seeded , then few single cells began to proliferate and formed the floating cell clones in the medium .

方法：大鼠经皮下注射Iso共10天以制备心肌纤维化模型，心肌线粒体以差速离心法分离。对照组仅注射无血清的培养液。

The control group was the model rats received only DMEM injection .

无血清细胞培养液的表面增强Raman及偏振光光谱研究

Research on Surface Enhanced Raman Spectrum ( SERS ) and Polarization Fluorescence Spectrum ( PFS ) of the Serum-free Cell Culture Solution

方法体外培养日龄3～5dSD乳鼠SC，收集无血清条件培养液，经超滤浓缩后制成冻干粉。

[ WT5HZ ] Methods Schwann cells of 3 ~ 5 day newborn mice were cultured , conditioned media without serum was collected , ultraspeed centrifugalized , and frozen dry .

骨髓内皮细胞无血清条件培养液对CFU-E和BFU-E生成的影响

Effects of serum free murine bone marrow endothelial cell conditioned medium on the growth of CFU-E and BFU-E

用于黄牛ICM分离的兔抗牛抗血清需在63.5℃灭活30min，且分离需在无血清的培养液中进行；

Anti-cattle rabbit serum for isolating cattle ICM should be inactivated at 63.5 C for 30 min and the isolation needs to be performed in serum-free medium ;

雪旺细胞无血清条件培养液中的神经营养蛋白活性

Activity of Neurotrophic Proteins Derived from Schwann Cell Serum-Free Conditioned Medium

奶牛卵丘细胞无血清体外培养液的优化

Optimization of Serum-free Media for Culturing in vitro of Cow Cumulus Cells

改良差速贴壁+无血清条件培养液纯化培养大鼠嗅鞘细胞

Purification and culture of rat olfactory ensheathing cells with modified differential adherence and serum-free conditioned medium

并在有血清和无血清的培养液中，比较血清对载体的转染效率的影响。在接近生理条件下进行共聚物的可降解性检测。

Transfection efficiency of copolymers in serum and serum free medium was determined by assay of transfection efficiency too .