无血清培养基

方法：在WilliamsE无血清培养基中，建立离体人头皮毛囊培养模型；

Methods We established the model of human hair follicle in vitro in Williams E serum-free medium .

氨基酸对中华仓鼠卵巢（CHO）细胞在无血清培养基中的作用

Effects of Amino Acids on CHO Cells for Long-term Culture in Serum-free Medium

血管内皮生长因子（VascularendothelialgrowthfactorVEGF）最初是从体外培养的豚鼠肝癌细胞无血清培养基中发现的。

Vascular endothelial growth factor ( VEGF ) was Originally found in the conditioned medium of guinea pig line 10 tumor cells grown in vitro .

无血清培养基孵育MHCC97-H细胞后，MMP-9和F型肌动蛋白主要位于细胞的核周池；

MMP-9 and F-actin were mainly localized in the perinuclear pool when the cells were incubated with serum-free medium .

目的对无血清培养基与含血清培养基培养CHO细胞进行比较。

Objective To evaluate the growth of CHO cells in serum-free and serum-containing media .

结论1、无血清培养基培养细胞时，VEGF可延长A549细胞的存活时间。

Exogenous VEGF could prolong survival of A549 human lung cancer cells cultured cells in serum-free medium . 2 .

结果显示，EGF和BPE在本实验所建立的无血清培养基中对培养表皮细胞DNA合成有明显的促进作用（P＜0.01）；

EGF and BPE were shown to promote the DNA synthesis of cultured epidermal cells significantly ( P < 0 01 ) .

Raji细胞在无血清培养基上的生长研究

Study on Serum-free Medium for Growth of Raji Cells

方法1.取对数生长期的C6胶质瘤细胞接种于无血清培养基，获得肿瘤干细胞。

Logarithmic growth phase of C6 glioma cells were seeded in serum-free medium in order to acquire BTSCs .

用于生产rHuEPO的无血清培养基的研究

Study on a serum free medium used for production of rHuEPO

用无血清培养基在填充床生物反应器生产rHuEPO

Production of rHuEPO with a Serum free Medium in Packed Bed Bioreactor

原代培养细胞在无血清培养基中生长2～3d后，大部分细胞死亡，只有少数以单细胞形式悬浮生长。

Most of the primary culture cells were dead after cultured in serum-free medium for two or three days , most of the cells died , only a few of single cells survived in suspension .

在无血清培养基中加入牛血清白蛋白（BSA）2mg／mL作为血清替代物，加入各种刺激因子进行实验。

Add bovine serum albumin ( BSA , 2 mg / ml ) as surrogate of serum before the addition of stimulators . 2 .

方法：取手术中切除的多余粘膜，分别用胰酶和Dispase分离后经胰酶消化，接种无血清培养基。

Methods : OMEC were primarily cultured with trypsin or dispase digestion .

方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。

Methods : Culture CHO cells in serum-free media and then compare the maintainance time , morphological changes and hemagglutination titers of the cells in culture supernatant .

简化无血清培养基分离U2-OS人骨肉瘤细胞系肿瘤干细胞

Cancer stem cells from human osteosarcoma cell line U2-OS in a serum-free medium

目的研究应用无血清培养基悬浮法培养C6胶质瘤细胞系的方法；并分离该细胞系中的脑肿瘤干细胞，观察其生长方式和分化特征。

Objectives : To study whether the C6 rat glioma cell line contains brain tumor stem-like cells and its growing pattern in serum-free medium supplemented with mitogens .

方法用含GMCSF和IL4的无血清培养基AIMV体外培养慢性乙肝病人外周血单个核细胞，获得树突状细胞。

Methods Peripheral blood monocytes ( PBMC ) were isolated from patients with chronic hepatitis B. DCs were generated by culturing PBMC with AIM V including GM CSF and IL 4 in vitro .

脐动脉环和脐静脉环在胶原凝胶和DMEM/F12（1：1）无血清培养基中生长良好，CD34免疫组织化学鉴定其为新生的血管。

Umbilical artery rings and umbilical vein rings embedded in three-dimensional collagen gels in DMEM / F12 ( 1:1 ) serum-free medium grew well . The new outgrows were positive by CD34 immunohistochemistry .

本研究选用SD大鼠背部皮肤毛囊和触须毛囊，在Williams'E无血清培养基中进行体外游离培养，建立了体外毛发毒性评价系统。

In this study , an assessment system of chemicals toxicity to hair in vitro was established by means of cultivating hair follicle of dorsal skin and vibrissa of SD rats in vitro in Williams'E media without serum .

细菌培养皿上用无血清培养基培养形成胚胎体5d后，约85%胚胎干细胞分化为Nestin阳性的神经前体细胞。

About 85 % of mouse ESCs were differentiated into Nestin-positive NPCs 5 days after the embryoid bodies formed in the bacterial dishes and cultured in the N2 serum-free medium .

结论：在离体培养人头皮毛囊时，WilliamsE无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；

Conclusion When we culture human hair follicle in vitro , Williams E serum-free medium is a suitable choice . This model is a fine model also , which is used for screening drug that may accelerate or inhibit the hair growth .

细胞培养：新生Wistar大鼠嗅球（OB）做OEG原代培养，然后利用无血清培养基添加一些促进生长的微量物质做纯化培养，为OEG提供了合适的生长环境而大量增殖。

Cell culture : The primary OEG was cultivated with the olfactory bulb of Wistar neonate rats , and then the purification was done by free serum medium .

方法：（1）建立用重组人GM-CSF、重组人IL-4、重组人IFN-α等细胞因子诱生，无血清培养基AIM-V培养慢性乙型肝炎患者、健康献血者外周血单个核细胞的方法。

Method : ( 1 ) Invitro culture PBMC separated from patients infected with CHB and the health , using substrate AIM-V and GM-CSF 、 rhIL-4 、 rhIFN - α .

CHO-GS细胞无血清培养基的优化：（1）氨基酸浓度的优化。

Design of a serum-free medium for CHO-GS cells . ( 1 ) Optimization of amino acids concentration .

因此，针对表达TNFR-Fc抗体融合蛋白的CHO-GS细胞，设计一种成本较低的无血清培养基，并且建立经济高效的流加培养工艺，对于TNFR-Fc抗体融合蛋白成功迈向产业化很重要。

Thus , it was important to design a low cost serum-free medium and develop an economic and efficient fed-batch process for TNFR-Fc industrialization . 1 .

采用低浓度血清培养基联合有丝分裂抑制剂培养，或者用高浓度血清培养基接种后换用含有血清替代物的无血清培养基维持生长，都可以成功培养GGCs和SGCs。

Both the medium with low concentration of serum and mitotic inhibitor and the serum-free medium supplemented with a substitute of serum are able to maintain the growth of GGCs and SGCs in vitro .

方法：对酶消化分离获得的表皮细胞，应用Ⅳ型胶原快速粘附法进行分选，并以角质细胞无血清培养基（K-SFM）进行培养，观察细胞生长状况及克隆形成情况；

Methods : The ESC digested and differentiated by enzyme were selected by means of type ⅳ collagen adhesion and cultured in keratinocyte serum free medium ( K-SFM ) to observe cell growth status and cloning formation .

无血清培养基中成釉细胞瘤细胞的生长特性

Growth Characteristic of Ameloblastoma cells Cultured in Serum-Free Medium in vitro

无血清培养基培养乳猪肝细胞的效果

Serum - free medium for culture of suckling pig hepatocytes