无菌培养

研究了白玉凤尾蕨（PterisCretica‘Albo-lineata’）孢子的无菌培养和常规繁殖方法。

The spore sterile culture and routine propagation of Pteris cretica'Albo-lineata'were studied respectively .

研究了西南凤尾蕨的发育过程和孢子的无菌培养方法。

The development process and spore sterile culture of Pteris wallichiana Agardh .

蕙兰CYMBIDIUMFABERIROLFE种子无菌培养的研究

Study on seed aseptic culture of Cymbidium faberi Rolfe

将棉花种仁无菌培养40～60h，制备茎尖分生组织，培养在无激素MSB培养基上，直接再生完整植株。

The apical meristems were excised from the seedlings of 40 ~ 60 hours and cultured on the MSB medium without / with hormone or active charcoal .

以无菌培养的原生动物纤毛虫瞬目虫（Glaucomascintillans）为材料，比较它们对数生长期和衰老期的酯酶同工酶，酸性磷酸酶同工酶，苹果酸脱氢酶同工酶及乳酸脱氢酶同工酶的差异。

The isoenzymes of esterases , acid phosphatase , malate dehydrogenase and lactate dehydrogenase of axenic culture of Glaucoma scintillans in log phase and in aging phase were studied by means of polyacrylamide electrophoresis .

无菌培养的建立：切取1cm左右的叶片，采取二次灭菌法提高成功率，接种于改进的培养基上。

Establishment of aseptic explant culture system : About 1 cm long blade was cut ; and the twice sterilization method was adopted to improve the sterilized efficiency and survival rate .

珍稀濒危植物&中华水韭孢子的无菌培养

Aseptic Culture of Spores in the Rare and Endangered Plant-Isoetes sinensis

达到了无菌培养和细胞壁再生。

Axenic cultures were obtained and cell wall regenerated during subsequent culture .

植物氨基酸营养研究中的无菌培养技术

Sterile Culture Methods Used in the Study of Plant Nutrition of Amino Acids

兰花种子的发芽及其无菌培养

The seed germination and sterile culture of orchids

大叶黑桫椤孢子的无菌培养

Spore Sterile Culture in Alsophila gigantea var. gigantea

盐藻的无菌培养及其抗生素标记的选择

Culture Condition and Selective Marker of Dunaliella salina

重楼属植物初代培养过程中无菌培养物的建立大袋无菌包装

Establishment of Aseptic Culture Material in the Initiation Culture of Paris Process of Aseptic Bag Packaging

无菌培养条件下不同基因型小麦耐盐性及其生理机制研究

Study on salt tolerance of different winter wheat genotypes and their physiological mechanism under sterile condition

水稻有机营养研究中完全及局部无菌培养方法探讨

Studies on totally and partially enclosed sterile culture methods used in the research of rice organic nutrition

本研究还以奥地利黑松种子在无菌培养基和营养土中培养的实生苗带子叶顶芽为外植体诱导丛生芽。

The study also used the plantlets of sterile seedlings and seedlings in soil to induce tufted buds .

取得了如下研究成果：1.建立了无菌培养技术体系。

The results were as followed : 1 . The technological system of asepsis culture had been established .

因此，在植物有机营养研究中采用局部无菌培养方法是可行的。

So partially enclosed sterile method can be used in the research of plant organic nutrition for its superiority and feasibility .

在无菌培养条件下，研究了天麻授粉后不同天数种子的萌发率；并进行了果实、种子大小以及种皮中营养物质的观测。

The percentages of germination of the seeds of Gastrodia elata on different days after pollination were studied under sterile condition .

供试所有种类弯孢霉的无菌培养滤液，可抑制玉米种子根的伸长，证明这4种弯孢霉在人工培养过程中都可产生致病毒素。

Some Curvularia species could produce pigment during culture , and their culture filtrate could inhibit the root growth of corn .

用此无菌培养试验法进行的水稻有机氮营养试验证明，水稻能直接吸收根际营养液中的氨基酸。

Results from sterile experiment for rice organic N nutrition showed that amino acid could be directly taken up by plants .

体外无菌培养的阴道毛滴虫加入蜂胶溶剂，观察不同作用时间、不同蜂胶浓度和蜂胶在不同滴虫密度下的杀虫效果。

Cultured T. vaginalis was used for the anti-trichomonas test at different times , concentrations of propolis and densities of the parasites .

95%以上的再生体形成根系，在无菌培养和温室中可形成完整植株。

More than 95 % of de novo regenerants formed roots and then intact plantlets under either sterile culture or greenhouse conditions .

为对象，研究了无菌培养条件下，氨基酸这一有机营养对它的营养效应，探讨了其作用的机理，并在田间条件下研究了氨基酸对黄瓜的营养效应。

Was used as the plant material in this paper . Nutrition of amino acids on vegetables was studied under sterile culture and in the field .

在无菌培养室内就水稻有机营养研究中水稻苗完全无菌及局部无菌培养方法作了比较研究。

Comparative studies between totally enclosed sterile culture method and partially enclosed sterile culture method used in the research of rice organic nutrition were conducted in sterile chamber .

以种子无菌培养进行繁殖，为研究植物试管条件下的生理生态提供了可能，同时为紫薇繁殖增加了新途径。

It was possible that sterile culture of seeds for the study of plants physiological and ecology in vitro conditions . And there was a new method to propagating .

本文研究了水稻幼穗在无菌培养和添加外源激素作用下，由颖花直接培养成植株的全部发育过程。

It deals with the developmental process through which the spikelets in the young panicles regenerated plants under the condition of sterile culture and the effect of hormone added .

通过对澳洲青苹茎尖培养及植株再生体系中无菌培养物的建立、初代培养、继代培养、生根培养和移栽五个技术环节的研究，建立了澳洲青苹组织培养快速繁殖技术体系。

The tissue culture of Granny Smith apple was studied in this paper from sterile culture , primary culture , subculture , rooting culture to transplantation . A rapid propagation method was established .

随着培养时间的延长，电化学阻抗值在无菌培养基中先降低而后升高，而在有菌培养基中一直降低，表明硫酸盐还原菌对304不锈钢的腐蚀起到了促进作用。

With the growth of the culture time , the value of electrochemical impedance in the medium without bacteria decreased at first , then rose , while in the medium included with bacteria fell at all times . It indicated that SRB accelerated the corrosion of stainless steel .

无菌操作培养人外周血淋巴细胞24h后，各组分别加入各浓度As2O3溶液及（或）姜黄素溶液。

The human peripheral blood lymphocyte be cultivated by aseptic technique approach for 24 hours . All groups be given different density As_2O_3 solution or curcumine solution .