尾芽

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

Suppression subtractive hybridization ( SSH ) cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp ( Carassius auratus gibelio ) .

分别取囊胚期胚胎和尾芽期胚胎各192和960个PCR阳性克隆进行斑点杂交，得到15个囊胚期和131个尾芽期的斑点杂交阳性克隆。

One hundred and ninety two and 960 PCR positive clones were respectively selected to perform dot blot tests ; 15 and 131 dot blot positive clones were obtained from SSH plasmid libraries in the blastula and tail bud stage embryos .

对原肠期739个和尾芽期816个PCR阳性克隆进行斑点杂交，得到72个原肠期和98个尾芽期斑点杂交阳性克隆。

739 and 816 PCR positive clones were respectively selected to perform dot blot , and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the SSH plasmid libraries specific for gastrula embryos and tail bud embryos .

本文采用活性电泳方法，对黑斑蛙（Rananigromaculata）早期胚胎发育过程中蛋白酶活性进行了研究.结果如下：尾芽期以前未检测到蛋白酶活性；

The G-PAGE was used to analyze the proteinase activity of Rana nigromaculata during early embryonic development . The results showed that : ① Before tail bud stage , there was almost no proteinase activity ;

在胚胎发育过程中，牙鲆各期胚胎对玻璃化液的适应能力不同，尾芽期胚胎较其他时期耐受力强。

Embryos at tail bud stage are more tolerant to vitrification solutions .

方法本研究在中国林蛙胚胎发育的尾芽期，用化学、物理和激素因子进行技术处理。

Method This study is aimed at the technical treatment with chemical , physical and hormone factors during tail bud period in the process of embryonic development .

结论：激光照射对尾芽胚双向电泳蛋白质谱有明显影响，蛋白质点的变化与激光能量密度相关。

Conclusion : Protein pattern of dimensional electrophoresis of tail bud embryos raried obviously after laser irradiation and there were relationships between protein pattern and laser energy density .

染色体绝对长度由囊胚列原肠晚期逐渐增长，神经胚期开始下降，尾芽期显著缩短。

The chromosome length increases gradually as the embryos proceed from blastulae to late gastrulae , but begin to reduce in neurulae and drastically shorten in tail-bud embryos .