尼龙膜
- 名nylon membrane
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第四,把有辐射能的或有色的探剂加到尼龙膜上就会制出一个所谓的DNA指纹图案。
Fourthly , adding radioactive or colored probes to the nylon membrane produces a pattern called the DNA fingerprint .
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采用高速打印或光刻合成技术可在硅片、玻璃或尼龙膜上制造DNA微阵列。
DNA microarray or gene chip can be fabricated on silica wafer , glass slide and nylon membrane by high throughput printing or light directed combinatorial chemistry .
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这些克隆的PCR产物经过浓缩后点制到尼龙膜上。
PCR products of these clones were concentrated and spotted on membrane array .
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5种性病病原体的PCR扩增产物,与膜上点有5种探针的尼龙膜杂交,结果在各自特异性探针的位置上出现杂交斑点;
And PCR products were hybridized with the species-specific probe respectively on nylon membrane .
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提取毛冠鹿高分子量基因组DNA,HindⅢ酶切毛冠鹿基因组DNA,将酶切后的基因组DNA转移至尼龙膜。
The genomic DNAs were extracted from tufted deer and were digested by Hind III respectively .
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将文库菌落印迹至尼龙膜,分区培养提取质粒DNA,建立基因池,并分别转染NIH/3T3细胞。
The plasmids in cDNA library and in gene pools were extracted and NIH / 3T3 cells were transfected respectively .
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结论在同一张尼龙膜上检测HBV基因型及YMDD变异,经济实惠、准确快捷,且易于开展。
Conclusion Detection of HBV genotypes and YMDD mutations together were economical , exact , rapid and easy .
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目标细胞的cDNA样品用同位素标记时,尼龙膜cDNA阵列对杂交信号探测的灵敏度高、线性范围大,对低表达量的cDNA也能进行有效分析。
With radiolabeled cDNA sample , nylon filter cDNA microarrays have high sensitivity and wide linear range of hybridization signals , so it can be employed to detect many important low abundance cDNAs .
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其次将尼龙膜剪切成大小合适的膜条,将多个样品DNA固定于膜条上,然后膜条与探针在2ml离心管中与探针杂交、检测获得杂交信号。
After clipping the nylon membrane into strips , the DNA of multi-samples is fixed on the strips , hybridized with probes and the hybridization signal is detected in 2ml microtubes .
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然后将探针与微阵列尼龙膜杂交,高严谨度洗涤后分析杂交图谱。
After a high - stringency wash , the hybridization pattern was analyzed .
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双向拉伸尼龙膜用树脂的开发现状
Development Situation of Resin for Biaxially Stretched Nylon Films
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用于亲和分离的尼龙膜的改性
Modification of nylon membrane for affinity purification
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每种探剂有选择地插在尼龙膜上仅一个或两个特定的地方。
Each probe typically sticks in only one or two specific places on the nylon membrane .
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玻片灵敏度为92.4%,尼龙膜灵敏度87.3%,χ2检验差异无显着性(P>0.05)。
The sensitivity of slide was 92.4 % , and the nylon membrane was 87.3 % ; no significant differences were found in χ 2 test ( P > 0.05 ) .
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超临界CO2对尼龙66膜的改性
Modification of Nylon 66 Membrane with Supercritical CO_2
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用新型尼龙亲和膜纯化人血浆蛋白
Purification of Proteins in Human Plasma with New Nylon Affinity Membranes
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尼龙-6膜与丙烯酸接枝共聚合的研究
Studies on grafting copolymerization of nylon-6 membrane with acrylic acid
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新型粮食储藏膜&尼龙复合膜
New grain store film & five layers coextrusion nylon film
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尼龙6膜固定化生物催化剂及其应用
Nylon 6 Membrane Immobilized Biology Catalyst and Its Applications
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硝酸体系尼龙微孔膜
Nylon - microporous membrane with nitric acid system
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尼龙肠衣膜的改性研究
Study of modification of nylon casing film
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支撑尼龙66微滤膜的性质及其褶筒型滤芯在纯水制备上的初步应用
The properties of supported nylon 66 microfiltration membrane and the initial application of its pleated cartridge filter core to making pure water
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介绍了PE和尼龙共挤出复合膜的结构性能以及在粮食储藏中的应用,并与传统材料PVC膜和PE膜进行对比
The structure and properties of coextrusion nylon / polyethylene composite film and its application for grain store were introduced . BLOWING FILM TECHNOLOGY OF MULTI-LAYER COEXTRUSION
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接枝密度随接枝率的增加而增加,当接枝率达到一定程度时,接枝密度不再变化,计算得到尼龙-6接枝膜的最大接枝密度为51.3%。
The grafting density did not change when grafting yield reached a definite degree . The maximum grafting density of grafting membrane is 51.3 % .
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本文研究了凝胶介质为醇/水溶液的情况下,在相转换法制备非对称尼龙6,6膜过程中,若干因素对凝胶速度及膜结构的影响。
Some factors affecting gelation rate and membrane structure of asymmetric nylon 6 , 6 membrane formed through phase inversion using aqueous alcohol solution as the gelation medium were studied .