对数生长期

实验结果表明，费氏丙酸杆菌菌株HZ-P-35在改良MRS培养基中厌氧培养时生长较为缓慢，96h左右对数生长期结束；

The following are the results : Strain HZ-P-35 has a slow growth in a modified MRS medium with a 96h time course of logarithmic growth phase .

取处于对数生长期的A549细胞，以0.25%的胰酶液进行消化，PBS洗涤，用不含血清的DMEM培养基稀释，以血细胞计数板进行计数，调整细胞密度为1×107/ml。

Digest the A549 cells in logarithmic growth phase with 0.25 % trypsin and dilute them with serum-free DMEM dilution medium to a cell density of 1 × 107 / ml after counting with CBC board .

悬浮液pH值的变化在对数生长期同生长曲线一致。

PH value of suspension cultural medium was in according with the calli growth curve .

通过不同生长阶段菌体的DNA提取结果，丝状真菌DNA的提取应选在其对数生长期为好。

The study , DNA extraction at different growth phase , shows extraction of filamentous fungi DNA should be at logarithmic phase .

MTT法于对数生长期筛选丹酚酸B的有效浓度；

Then , by using MTT assay , select the drugs efficacious concentrations in logarithmic growth phases of cell .

培养2h即进入对数生长期，8h后进入菌体衰亡期，没有明显的稳定期；

After 2 hours culture , the bacterium get a post exponential growth phase .

测定微生物生长曲线，得到该种微生物的对数生长期在2d左右，为微生物的快速解毒提供了理论基础。

The logarithm growth curve of microbe is determined and its growth period is two days , which provides the theoretical base of quick detoxification .

方法1.取对数生长期的C6胶质瘤细胞接种于无血清培养基，获得肿瘤干细胞。

Logarithmic growth phase of C6 glioma cells were seeded in serum-free medium in order to acquire BTSCs .

细胞活力测定：MTT法绘制细胞增殖率生长曲线，取处于对数生长期的4-6代细胞进行试验。

Determination of cell viability : MTT proliferation growth curve , taken in the logarithmic phase of the experiment , 4-6 cells . 3 .

细胞接种后第1个24h为细胞潜伏适应期，第2~7个24h生长曲线基本为线性曲线，是细胞的对数生长期。

The first 24 hours was the lag phase , the second to seventh 24 hours were the Log phase by the growth curve .

MTT比色实验：选择在对数生长期生长良好的细胞，制成单细胞悬液，接种细胞于96孔培养板中，其中设空白对照组不加受试细胞。

MTT assay : The suspended cells obtained from the exponential phase of growth Cells were incubated into 96 ~ well Plates . Setting up blank group added no hepatoma carcinoma cell .

方法：将B16细胞株黑色素瘤对数生长期细胞接种于ICR小鼠足垫、皮下、腹腔和尾静脉等部位，建立移植瘤模型和肺部转移瘤模型。

Methods : Inoculating B16 Melanoma cells in ICR mice 's feet , back , abdomen and caudal vein , to establish transplanted and metastatic melanoma models .

MTT法测定细胞增殖速度，绘制细胞生长曲线，关节软骨细胞传代后细胞生长呈现旺盛增殖，在短暂的平台期后即进入快速生长的对数生长期。

We used MTT to detect cell proliferation , cell growth curve . Passage cells of articular cartilage cells showing strong growth and proliferation , and passed into the logarithmic phase after After a brief plateau period .

方法：使用对数生长期的人结肠癌细胞（HT-29）于裸鼠皮下接种成瘤后原位种植。

METHODS : The cell line HT-29 in log phase of human colorectal cancer was implanted subcutaneously in nude mice and subsequently the subcutaneous implanted tumor was orthotopically implanted .

经消化传代后的第1、3、10代细胞均经历24～48h的潜伏期，此后为3～5d对数生长期，逐渐进入生长平台期。14代以前具有活跃的增殖能力。

After digestion , the cells at passages 1 , 3 , 10 experienced the latent period ( 24-48 hours ), logarithmic phase ( 3-5 days ) and growth platform phase .

5种原料提取多糖对双歧杆菌的增殖作用：菌体在对数生长期生长速率常数（R）提高135%～85%，世代时间（G）缩短56%～44%。

The multiplication of bifidobacterium functioned by polysaccharose extracted from five kinds of vegetal was that the growth rate constant ( R ) is raised by 135 % ~ 85 % and the generation time ( G ) is reduced 56 % ~ 44 % in logarithmic phase .

且在本实验产生辐射防护作用的浓度范围内，C3对培养的对数生长期AHH-1细胞存活率几乎无影响，提示该类化合物有作为新型辐射防护剂的研究前景。

C3 does little harm on the survival rate of AHH-1 cell which suggests that C3 as a novel promising radio-protectant deserve to be fuaher investigated .

A549肺腺癌细胞对数生长期群体倍增时间在蒿甲醚作用后（20.7±0.5）h，对照组为（32.2±0.3）h，两组比较有显著性差异（P<0.01）。

The population doubling time in logarithmic growth phase in the artemether treatment group was ( 20.7 ± 0.5 ) h compared to ( 32.2 ± 0.3 ) h in the control group . The difference between two groups was statistically significant ( P < 0.01 ) .

随后的RT-PCR分析发现，与野生株相比，缺失株在对数生长期和稳定期溶血素基因表达没有显著变化，而丝氨酸蛋白酶基因在对数生长期表达较高，在稳定期显著降低。

But the analysis of real time-PCR showed that , compared with the wild strain , expression of hemolytic gene had no significant difference , while expression of serine protease gene was higher at exponential stage and lower at stationary stage in the luxS-deleted mutant .

细胞在对数生长期的倍增时间为30h左右；约传10代后进入衰退期；

The doubling time of the cells in logarithmic growth period was around 30 hours , the cells entered decline period after expanding for 10 generations .

以无菌培养的原生动物纤毛虫瞬目虫（Glaucomascintillans）为材料，比较它们对数生长期和衰老期的酯酶同工酶，酸性磷酸酶同工酶，苹果酸脱氢酶同工酶及乳酸脱氢酶同工酶的差异。

The isoenzymes of esterases , acid phosphatase , malate dehydrogenase and lactate dehydrogenase of axenic culture of Glaucoma scintillans in log phase and in aging phase were studied by means of polyacrylamide electrophoresis .

获得优化培养条件：诱导温度为34℃，IPTG量为0.4mM，诱导时机为对数生长期中期，诱导后表达6hour终止。

The optimized culture conditions were : induction temperature 34 ℃, IPTG concentration 0.4mM , induction timing mid-exponential growth phase , induction time 6 hours .

实验结果表明：使用对数生长期早期的细胞、蜗牛酶浓度1%，30°C处理50～60min，山梨醇为渗透压稳定剂，有利于原生质体制备和再生。

Results showed that it was beneficial to protoplast formation and regeneration under the conditions of using early exponential phase cell , 1 % snail enzyme , at 30 ° C , stirring slowly for 60 minutes and sorbitol as osmotic stabilizer .

细胞增殖曲线显示猪成骨样细胞在播种3d后进入对数生长期，15d左右达到生长峰值U经细胞倍增时间公式计算猪成骨样细胞倍增时间为67.2h。

Cell growth curve showed that osteoblast-like cells from pigs entered logarithmic phase at day 3 after seeding , reached peak growth value at about day 15 . The doubling time of osteoblast-like cells from pigs was 67.2 hours calculated with cell doubling time formula .

大鼠心肌细胞（H9C2）经体外培养扩增，使用对数生长期细胞做实验处理。

Rat myocardial cells ( H9C2 ) by in vitro culture , using the logarithmic growth phase cells as experimental treatment .

采用细胞体外培养的方法，在卵巢颗粒细胞对数生长期，对F-2毒素的繁殖遗传毒性和V-E对其的解毒作用进行了研究。

During logarithmic phase of cultivated ovary granulose cell , experiments were carried out to investigate the genetically procreation toxicity of F-2 toxin and the detoxification of V-E.

方法：取对数生长期乳腺癌细胞系MCF-7、SK-BR-3和BT-474，实验组以终浓度为10、100、500、1000U/ml的IFN-γ诱导培养48h，对照组加入等量不含IFN-γ的培养液。

Methods : The human breast cancer cell lines MCF-7 , SK-BR-3 and BT-474 were in logarithmic growth phase and cultured in the presence of 10,100,500 or 1000U / ml interferon - γ for 48 hours .

发酵罐高密度培养对数生长期由6h提高到14h，延长8h，最大活菌数从3.9×10~8cfu/mL提高到4.6×10~9cfu/mL，提高10.8倍。

Logarithmic phase of high-density culture prolonged 8 hours from 6 to 14 . Max viable count increased from 3.9 × 10 ~ 8cfu / mL to 4.6 × 10 ~ 9cfu / mL , more than 10.8 times .

方法将对数生长期人肝细胞肝癌FHCC98制成细胞悬液（1×107/ml），取0.2ml分别注射于16只裸鼠胁部皮下（1处/只），建立裸鼠肝癌动物模型。

Methods FHCC-98 , a human HCC cell line , was implanted subcutaneously to the costal regions of 16 nude mice by injection ( 1 × 107 / ml , 0.2 ml , respectively ) .

另外，ΔntrC在菌体形态变化速度上显著不同于野生菌，其形态在对数生长期几乎全是杆状。

In addition , the cell morphological change rate was also different from that of wild type strain and it was almost in rod type in exponential phase .