多功能酶

浅谈多功能酶

Discuss multifunctional enzyme superficially

组织型谷氨酰胺转氨酶是一种多功能酶，不仅可以催化多种蛋白质形成交联分子稳定细胞外基质，而且可以调节肿瘤生长、细胞分化、细胞凋亡和细胞黏附。

Tissue transglutaminase is a multifunctional enzyme which is involved in the regulation of cell growth , differentiation and apoptosis .

研究方法：选用原代培养的新生SD大鼠心肌细胞，应用全波长多功能酶标仪测量系统测定心肌细胞内pH值；用westernblot检测心肌细胞ERK的磷酸化水平。

Methods : The study was performed with primary cultured neonatal rat cardiomyocytes . Intracellular pH was measured using microplate reader of multi-wavelength measurement system ; Expression of phosphorylated ERK protein was determined by western blot . Results : 1 .

PEPCase是一个在植物中广泛存在的多功能酶。

Phosphoenolpyruvate carboxylase ( PEPC ase ) is a ubiquitous and multiple functional enzyme in higher plants .

多功能酶的预测及结构功能模式分析

Prediction and Feature Analyses of Multi-Functional Enzymes

多功能酶由于其特殊的性质成为了酶分类问题当中非常棘手的异类。

Multifunction enzyme has become a very tricky heterogeneous because of its special properties when facing the problem of enzyme classification .

脂肪酸合酶（FAS）是一种多功能复合酶，是体内合成脂肪途径中的一个关键酶。

Fatty acid synthase ( Fas ) is a multifunctional composite enzyme , is synthesized in vivo fat pathway of a key enzyme .

脑型一氧化氮合成酶的钙调蛋白结合区的表达及活性鉴定钙调素（Calmodulin，CaM）是一种多功能的酶调节物。

Expression of Calmodulin binding Domain of Neuronal Nitric Synthase and Its Binding Activity to Calmodulin The interaction between calmodulin and calmodulin binding domain of cell membrane can be characterized by affinity constant .

每浓度设5个重复，作用24h后用PBS洗涤，加入EB染色，用多功能荧光酶标仪检测细胞的GFP和EB荧光强度。

Each concentration was repeated for five times . After 24 hours of incubation , the cells were washed with PBS and stained with EB , fluorescence intensity of GFP and EB was measured by the fluorescence microplate reader .

结合增效研究，推测苹果黄蚜杭性机制以微粒体多功能氧化酶为主体酶系。

Therefore , the activities of detoxification enzyme systems were important mechanism of the green apple aphid resistance to insecticides .