噬菌斑

用定量PCR从杂交阳性融合噬菌斑中分离候选插入片段

Isolating candidate inserted fragment from positive fused phage clones using quantitative PCR

以栽培大豆7S贮藏蛋白a′-cDNA作探针，用噬菌斑原位杂交法从文库中筛选出一个阳性克隆。

The library was screened by using α ' - cDNA of cultivated soybean storage protein as a probe and one positive clone was obtained .

通过Phage-ELISA和噬菌斑印迹法初步证明了亲和噬菌体表达蛋白与病毒之间的相互作用。

The interaction between IBDV and the expression protein was proofed preliminarily by Phage-ELISA and Plaque lift .

噬菌体颗粒大小与其遗传控制的噬菌斑大小的关系

Study on interrelationship between size of bacteriophage particles and size of plaques

噬菌斑电子图像的计算机处理及其自动计数

Plaque Electronic Image Segment and Automatic Counting by Computer

一种从培养皿上挑取阳性噬菌斑的改进方法

An improved Method to Screen Positive Colonies from Plate

牙齿上留有很多噬菌斑

Heavy plaque left on the teeth

噬菌斑的概率分布及单噬菌体增殖研究法的实验设计

The probability distribution of plaques and an experimental design for the study of a single individual bacteriophage multiplication

二价阳离子Ca2+或Mg2+对促进宿主细胞裂解和提高噬菌斑的形成是必需的。

The presence of calcium or magnesium ions was necessary to accelerate cell lysis and improve plaque formation .

这四株噬菌斑形态不同的噬菌体，对紫外线的敏感性和在20种血清型的32个菌株中的寄主范围各不相同，证明它们是四种不同类型的噬菌体。

They are different in plaque morphology , UV sensitivity , and host range in 32 tested strains .

根据噬菌体形态、噬菌斑类型、对抗性菌株的裂解能力，选择一批制备抗血清，借交叉中和试验将63株噬菌体分成12个血清型。

By using anti-phage sera prepared from 7 phages , the 63 phages were divided into 12 serotypes by neutralization test .

本文报道了这7株温和性噬菌体的噬菌斑形态、噬菌体颗粒形态、溶原菌的稳定性和免疫性以及血清学特异性。

The morphological character of plaque and particle , the stability and immunity of lysogen , the serological specificity of these phages were studied .

刚开始分离得到的噬菌斑较小，经进一步的纯化后，其噬菌斑明显增大，对宿主菌的裂解能力增强。

The plaque is relatively small at the beginning , but after further purification , the plaque significantly increased and enhanced the ability of lysis to the host bacteria .

方法采用宿主范围测定、噬菌斑计数、负染色电镜观察、血清中和试验及血清交叉中和试验、一步生长实验等技术观察分离的噬菌体。

Methods The phage was identified by detection of the host range , calculation of phage plaque , negative staining for TEM , serological neutralization test and one step growth experiment .

随机挑取11个噬菌斑，用载体克隆位点两端的通用引物进行扩增，以检测所构建的文库的质量。

Then λ phage packaging reaction and library amplification were performed . Eleven plaques were randomly picked and tested using PCR method with universal primers derived from the sequence flanking the vector .

细胞密度影响试验表明细胞密度为4×108cells/mL时，噬菌斑形成单位数最高。随着细胞密度的逐渐增加或减少，噬菌斑形成单位数均呈下降趋势。

Experiments of the cell density showed that the plaque forming unit was the highest at 4 × 10 8 cells / mL , and with the increase or the decrease of the cell density , both of the plaque forming unit decreased gradually .