反向引物
fǎn xiànɡ yǐn wù
- reverse primer
反向引物
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而对于每个反向引物,在与11个不同的正向引物组合时,产生多态性的引物组合数则在2-11个之间,差异较大。
When each reverse primer was in combination with different forward primers , the number of polymorphic primer combinations ranged from two to eleven .
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设计1对正、反向引物btubf/btubr,对家蚕微孢子虫(镇江株)基因组DNA的β-微管蛋白(beta-tubulin)基因进行扩增,得到部分片段。
A beta-tubulin DNA fragment of the Zhenjiang strain of Nosema bombycis was obtained by primers btubf / btubr .
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对于每个正向引物,在与12个不同的反向引物组合时,产生多态性条带的引物组合数均在5-8个之间;
When each forward primer was combined with different reverse primers , the number of primer combinations that produced polymorphism ranged from five to eight .