化学法

化学法抑制米糠中脂酶活性及对出油率的影响

The restraint of hte activity of the lipase in rice bran by chemical approach and its effect on the efficiency of oil tapping

化学法糖基化存在区域选择性差、需要保护/脱保护步骤和环境不友好等诸多问题。

The conventional chemical approach is characterized by low regioselectivity , the requirement of protection / deprotection , and being environmentally unfriendly .

免疫组组织化学法检测增殖细胞核抗原（PCNA）的表达。

Proliferating cell nuclear antigen ( PCNA ) of transferred SMG was studied by immunohistochemistry .

分别用生物化学法和ELISA法检测巨噬细胞因子TNFα和IL-6的表达；

The pro duction of TNF - α and IL-6 were measured by bio-chemical method and ELISA res pectively ;

介孔纳米TiO2的超声化学法合成及其表征

Sonochemical synthesis and characterization of nano-scale mesoporous tio_2

免疫组织化学法及Western印迹法检测肾组织中WT1和Bcl-2蛋白质表达。

Immunohistochemistry and Western blot were used to examine the expression of WT1 and Bcl-2 gene products in renal tissue .

并将转基因肿瘤细胞接种于裸鼠体内，应用免疫组织化学法检测肿瘤内微血管密度（MVD），探讨MVD与肿瘤生长转移的关系。

Microvessel density ( MVD ) in xenografted tumors in nude mice was analyzed by immunohistochemistry .

通过免疫荧光细胞化学法检测神经纤丝（NF）的表达，进行神经元鉴定及纯度计算；

Neurofilament ( NF ) was detected by immunofluorescence staining to identify neurons and to calculate the purity of neurons .

福尔马林处理后2h，免疫组织化学法检测大鼠脊髓后角CFos蛋白的表达。

At 2 h after injection of formalin , the expression of C-fos protein in spinal dorsal cord was detected using immunohistochemical method .

我们用X射线荧光光谱滤纸片法分析了四个古代青铜兵器中的Cu、Pb、Sn含量，铜的测量结果与化学法一致。

We have measured the content of Cu , Pb and Sn in four samples of the ancient bronze weapon using X-ray fluorescence filter paper method .

化学法合成生物完全降解塑料PHB及单体3-羟基丁酸的研究进展

Advancement of chemical synthesis of completely biodegradable plastic PHB and its monomer 3 hydroxybutyric acid

文中介绍了激光化学法从CHCIF2中分离~（13）C同位素的机理、实验和验证、与其他分离方法的比较、以及最新进展。

This article describes the mechanism of separation of C-13 from CHCIF_2 by laser chemistry process , experiments of separation and check , the comparison with other separation processes and the latest deve (?) opment at present .

尽管已经进行了许多研究工作，其中化学法用H2O2－HCl、浓HNO3体系分离矿物中的晶格金。

They separated ionic gold from sulfides by chemical method of H 2O 2 HCl , conc .

采用间接免疫荧光细胞化学法和激光共聚焦显微镜进行连接蛋白Cx43定位的测定；

The localization of Cx43 proteins were performed by indirect immunofluorescence histochemical analysis and detected by confocal microscopy .

试验方案Ⅱ-黑液用化学法（HCl）去除木质素后进行厌氧发酵，Ⅱ号反应器内装软填料。

The second experiment is that the anaerobic digestion is carried out after lignin has been removed from the black liquor with chemical method .

5-HT受体亚型mRNAs在大鼠背根神经节和三叉神经节的表达&原位杂交组织化学法研究

Expression of 5-HT receptor subtype mRNAs in the dorsal root ganglion and trigeminal ganglion of the RAT-IN situ hybridization histochemical study

用免疫组织化学法检测第八因子相关抗原（FⅧAg）、VEGF在食管组织中的表达。对FⅧAg阳性血管进行微血管密度计数（MVD），对VEGF染色结果进行计算机图像定量分析。

The expression of VEGF and factor ⅷ related antigen ( F ⅷ Ag ) was detected by immunohistochemistry , and the microvessel density ( MVD ) was counted under F ⅷ Ag staining .

组织化学法显示：这些细胞具有AcP和NsE阳性反应性，呈PAS阳性反应以及其胞质含有被醛复红染色的颗粒。

By histochemistry , the cells were found to present AcP , NsE and PAS positive reactions and contain granules stained by aldehyde-fuchsin in the cytoplasm .

方法采用NADPH-黄递酶（NDP）组织化学法和整装铺片技术对烧伤大鼠小肠壁内NOS的活性及分布进行定量和定位研究。

Methods The activity and distribution of NOS were quantitatively and locationally studied by histochemical staining with NADPH-diaphorase ( NDP ), a marker for NOS , and whole mount preparation technique .

方法：以绿脓杆菌菌毛制剂，作用于体外培养的人PBMC，采用生物素-链霉亲和素（BSA）免疫细胞化学法，检测人PBMC表面CD2、CD25、CD69的表达。

Methods : The techniques of immunocytochemical method and biotin streptavidin ( BSA ) were applied for evaluation of expression rate of CD2 , CD25 and CD69 of human PBMC by PPA stimulation .

结果术后30d内，植片内神经溃变，同时宿主角膜实质神经出芽再生。神经溃变及再生的组织化学法评价

Results One month after PKP , the graft nerves degenerated and some stromal nerve trunks in host corneal tissue were sprouting . Histochemical Evaluation of Nervous Degeneration and Regeneration

用NADPHd组织化学法，检测局灶性脑缺血再灌注后缺血半暗区（额叶皮层）和中心区（顶叶皮层和尾壳核）NOS活性变化。

The changes of nitric oxide synthase activity in ischemic core area ( Parietal cortex and Caudoputamen ) and penumbral area ( Frontal cortex ) was examined after focal cerebral ischemia and reperfusion using NADPH d histochemistry technique .

4周后分别检测空腹血糖（FBG）、空腹血清胰岛素（FINS），免疫组织化学法检测胰岛PDX-1、AKT、Bcl-2、胰岛素的表达。原位检测胰岛细胞凋亡。

Islet cell apoptosis , fasting blood glucose ( FBG ) and fasting insulin level ( FINS ) were detected and expressions of PDX-1 , AKT , Bcl-2 and insulin in pancreatic islets were detected by immunohistochemical assay .

于术后6,24,48,72h和5d，采用免疫组织化学法检测各组脊髓组织凝血酶受体1表达。

Six hours , twenty-four hours , forty-eight hours , seventy-two hours and five days after operation , the spinal cord tissue was taken out to detect the PAR-1 expression by immunohistochemical method .

实验结束取肺组织，测定肺组织湿干比，采用流式细胞分析法检测肺组织细胞凋亡率和免疫组织化学法检测肺上皮细胞Bax、Bcl2、Fas/FasL蛋白表达的变化。

Alveolar cells apoptosis and Bax , Bcl-2 , Fas / FasL protein expression were studied By using flow-cytometry and immunocytochemistry techniques . Results The lung apoptosis cell number was increased in ischemia groups compared with control and sham group .

本文采用三聚氰胺和硼酸为原料（质量比2：1），经化学法合成B-C-N前驱物，再经过高温高压处理，合成立方B-C-N化合物。

In this paper , B-C-N precursor was prepared by a chemical process using boric acid and melamine as starting materials . Then cubic B-C-N compound was synthesized by HPHT treatment .

免疫细胞化学法测ICAM-1表达，丫啶橙荧光染色法和流式细胞仪测肝细胞凋亡率，评价Grz护肝作用。

Aminoguanidine was used to confirm the target of Grz action . Expression of intercellular adhesion molecular-1 ( ICAM-1 ) was determined by immunocytochemistry . Percentage of apoptosis was detected with fluorescence microscope and flow cytometer .

用免疫组织化学法检测不同时间点（4,7,11,15d）海马区NF-kB、I-kBα的蛋白表达，同时行为学观察和脑电图确定痫性发作。

Results : The protein expression of NF-kB and I-kB α was detected by using immunocytochemical stains at different time ( 4,7,11,15d ) . The seizures were determined by ethology and EEG .

用自体动脉血注入尾状核法建立大鼠脑出血模型，分别用免疫组织化学法和Evans蓝法检测大鼠脑出血后不同时间点血肿周围AQP-4和血脑屏障通透性的动态变化。

The intracerebral hemorrhage model in rats was established by injecting autologous arterial blood into caudate nucleus . Immunohistochemical method and Evans blue method were used respectively to detect the dynamic changes of AQP-4 around hematoma and the blood-brain barrier permeability in different time periods after intracerebral hemorrhage in rats .

结果表明，机械化学法可以制成超细的无定形混合粉体，将这种粉体在973K下煅烧5h可以获得纳米锰酸镧粉体。

The results indicate that an amorphous phase of mixtures can be efficiently activated by the mechanochemical process . Once such ground powder mixture being further annealed at a lower temperature of 973K for 5 hours , crystal LaMnO_3 with nanoscale particle size was thus formed .