动情期

非动情期SD大鼠子宫内膜异位症模型的建立

Establishment of Endometriosis Model in SD Rats during Non-estrus

给正常健康的青春期前雌性小鼠注射leptin可加速其性成熟出现，提前进入动情期。

After injecting healthy prepubescent female mice with leptin , they would advance sex maturation and enter estrous cycle sooner .

于动情期取血，测定血浆β-内啡肽、血清E2和P含量。

The rats ' blood was gotten when they were in heat period and the content of plasma β - endorphin (β - EP ), serum E_2 and P were measured .

方法：雌性SD大鼠60只，选择其动情期，取切5×5mm~2的自体子宫内膜组织片断种植于左侧腹壁上。

Method : Sixty female SD rats ' left uterine horn was resected in estrus , then implanted the self-endometrium fragments measuring 25mm ~ 2 on the right peritoneal wall .

动情期和动情间期则延长，31.80mg/kg组的动情间期持续时间明显比对照组短；各组动情周期异常率均高于对照组，在31.80m眺g组差异具有显著性。

Proestrus and metestrus prolonged significantly in 1.91mg/kg and 31.80mg/kg group than that of the control and the duration of estrus and diestrus was reduced .

术前一天给将要造模的大鼠皮下注射己烯雌酚，剂量为0.1mg·kg-1，使手术时大鼠统一处于动情期。

In order to let the rats under unified estrus , the rats were given subcutaneous injection of Diethylstilbestrol 0.1 mg kg-1 the day before surgery .

妊娠期子宫内膜组织TACEmRNA的表达显著高于未孕动情期（P0.05），且随着妊娠天数的增加呈现逐渐增强的趋势，到妊娠d4达高峰，以后渐降。

The expression of TACE mRNA in pregnant groups was higher than that in estrus stage group ( P0.05 ) and appeared an increased trend as time passing by , reaching the maximum at pregnancy d4.2 .

而小鼠动情期PCNA标记细胞（22%）最多，并主要分布在表面上皮（60%）。

But in mouse , the results suggested that the PCNA labelled index was the highest ( 22 % ) in endometrium of estrus and the labelled cells were distributed mainly in superficial epithelium ( 60 % ) .

研究方法：采用12月龄的，阴道细胞学表现动情期延长的Wistar雌性大鼠作为初老大鼠模型。

Methods : 12-months old Wistar female rats whose oestrus elongation were set as primary senile female rat model and randomized into model control group , tonifying kidney formula in high dosage , middle dosage and low dosage group .

IGF-IR的表达强度随孕天降低，孕5～6d达到最小值，孕8～10d又上升到动情期水平，与P及PR均呈显著性负相关（P<0.05）。

The intensity of IGF-IR expression in endometrium appeared like a trough and reached bottom during 5 ~ 7 day of pregnancy and showed negative significant correlation with P and PR ( P < 0.05 ) .

对照组未经干预，在动情期取子宫标本。

The uterus tissue of control group were collected at estrus without interferes .

侧脑室注射芹菜素对动情期雌性大鼠卵巢功能的影响

Effect of Lateral Ventricle Injection of Apigenin on Ovary Function of Female Rats during Estrus

动情期家兔输卵管上皮及其分泌活动的电镜观察

The Oviductal Epithelium of Estrus Rabbits And Its Secretory Activity : A Electron Microscopical study

棉酚的这种药理作用还与性周期有密切关系，对动情期输卵管的作用明显高于间情期。

The pharmacological effect of GAA was also shown to be relevant to sexual cycle .

取动情期大鼠子宫内膜片段移植于卵巢周围或腹壁内侧面。

The fragment of endometrium of estrous rat was transplanted around ovary or in abdominal wall .

用于指非人类哺乳动物；处于两个动情期之间的状态。

Applies to nonhuman mammals : a state or interval of sexual inactivity between two periods of estrus .

（指在一个繁殖期有几个发情周期的动物）不动情期。

( of animals that have several estrous cycles in one breeding season ) in a period of sexual inactivity .

动情期组分别增加70%、77%、41%和41%；

A group of estrus were increased by 70 % , 77 % , 41 % and 41 % , respectively .

动情期，基质中信号强，但颗粒细胞和黄体上水平弱的几乎看不见。

In estrous , stroma displayed high level , but granulosa cells and corpus luteum were revealed weak almost invisible levels .

结论自体移植法建立非动情期大鼠内异症模型同时给予雌激素支持异位内膜生长，造模结果理想，证实此法经济可靠，尤适用于同批大样品的研究。

Conclusion Establishment of endometriosis model by autologous transplantation on SD rats during non-estrus with estrogen was success and credible , which was especially suitable for large sample research .

蛋白免疫印迹技术也显示这两型蛋白在输卵管和子宫均表达，动情期和动情间期这两型蛋白在输卵管和子宫中的表达水平无显著性差异。

Western blotting showed that Prx ⅰ and ⅱ were expressed in the uterus and oviduct , and their levels of expression had no significant differences between diestrus and estrus . 3 .

方法：4月龄～6月龄雌性大鼠为正常对照组；10月龄～12月龄，阴道细胞学表现动情期延长的雌性大鼠作为初老大鼠模型。

Methods : 4 ~ 6 months old SD female rats were set to be normal control group and 10-12 months old SD female rats whose oestrus elongation were set as primary senile female rat model .

方法：1.收集动情周期各期和早孕d1～d7小鼠子宫内膜，共11组，每组10只小鼠。

Mouse endometria during estrus cycle and early pregnancy were gathered in the study . There were 11 groups , and each group contained 10 mice .

药理试验证明草苁蓉碱对中枢神经有特殊作用，能增强苯巴比妥的镇静和催眠作用，对未孕大鼠，可缩短动情的休止期，延长动情期和后期。

Experiment of pharmacology proved that boschniakine had special effect for central nervous system It could help the phenobarbital for its calming and mesmerizing effects .

结果：1.RT-PCR：动情周期及早孕期小鼠子宫内膜均有TACEmRNA表达。

RT-PCR : TACE mRNA was expressed in mouse endometrium during estrus cycle and early pregnancy .

小鼠动情周期和妊娠期子宫内膜中PCNA标记细胞分布及数量的变化

Distribution and Numerical Changes of PCNA Labelling Cells during Estrus Cycle and Pregnancy of Mouse

动情周期、妊娠期与产后大鼠离体子宫的肾上腺素能效应

Adrenergic effect of isolated rat uterus during oestrous cycle , pregnancy and postpartum

动情间期和动情期表达减弱；动情后期表达极低；

Low level expression at diestrus and estrus , and the less expression at metestrus ;

采用细胞涂片法观察大鼠阴道细胞形态，确定动情周期：接收期与非接收期。以旷场实验和悬尾实验两种行为学方法评价模型。

In the process , we observed the vagina cell shape to make sure the estrum of rats : receptive phases and non-receptive phases , and then estimated model rats by open-field test and tailing suspension test .

结果发现，动情前期和间情期Frizzled3相对表达水平最高，动情期和动情后期出现显著降低（P0.05）。

It was found that the relative expression level of Frizzled 3 was highest in proestrus and diestrus , significantly decreased in estrus and metestrus ( P0.05 ) .