凝块

从极少量人血凝块中快速提取基因组DNA的方法

Fast method of extracting DNA from very few human blood clots

结论：利用血凝块的融化液直接进行DNA提取是一种简便、实用的DNA提取方法。

Conclusion : DNA extraction with semi-frozen blood clot is easy and practical .

血凝块DNA提取方法探讨

A novel method for DNA extraction from blood clot

维生素E和阿斯匹林能驱除血凝块

Vitamin E and aspirin can drive the clot away

方法：利用血凝块融化液直接进行DNA提取，并通过血凝块融化液涂片、血凝块冰冻切片以及琼脂糖电泳鉴定所提DNA的质和量。

Methods : DNA was extracted directly from the fluid at the bottom of semi-frozen blood clot .

Ca~（2+）和pH值对凝乳酶活性及干酪凝块质构的影响

The effect of Calcium on Cheddar curd

随切割pH值的升高，凝块的硬度值显著增大（p<0.05）；

The firmness of curd increased significantly ( p < 0.05 ) with increase of cutting pH ;

冰片iv2.0mg·kg~（-1）能够减轻小鼠全血凝块重量（P＜0.01或P＜0.05）。

Kg ~ ( - 1 ) ( P0.01 or P0.05 ) . Whole blood clot of mice was cut down with Borneol iv 2.0 mg ?

22例IgM阳性患者中，从5例患者的血凝块中扩增得到特异性PCR产物。

Specific PCR product was obtained from blood clot of 5 of the 22 IgM-positive patients .

在标准加热温度和不同pH值条件下，测定经不同浓度的氯化钙处理后的凝块在不同凝乳时间下质构的差异；

Under the condition of standarded heating temperature and dissimilar pH , the differential TPA dealed with different Calcium concentration in different clotting time was found out .

加热终点温度对凝块产率和硬度影响显著（p<0.01），其次是升温速率，切割pH值影响较小；

Cooking out temperature had a significant ( p < 0.01 ) influence on yield and firmness of curd , the effect of cutting pH is smallest .

方法从人血凝块中提取基因组DNA，用聚合酶链反应扩增出APOE基因片段，用限制性内切酶CfoⅠ消化，经聚丙烯酰胺凝胶电泳分离，使之分型。

METHODS The genomic DNA was extracted from the blood clot and the related Apo E gene sequence was amplified by PCR .

结果显示脑血肿的病理变化过程为：红细胞悬液血液浓缩、红细胞聚集血凝块形成和收缩红细胞溶解HS形成及低蛋白血肿液。

It 's manifested that pathologic change process of ICH was RBC suspension ── hemoconcen tration ── clot formation and contraction ── RBC lysis - H-S formation and lower protein liquid .

结果评价三种血液标本类型，血凝块提取DNA的综合评分最高，其次为全血和WBC；

Results When three types of blood samples were assessed , the composite score about clotted blood was the most , whole blood secondly and WBC thirdly .

结果在SAH模型大鼠的蛛网膜下腔均发现大量血液或血凝块。

Results : Extensive blood or blood clot was found in subarachnoid spaces of all SAH model rats .

结果体外血浆凝块溶解时间，FDP在给药组之间，给药组与对照之间无明显差异；

The results showed that there were no significant differences of PCDTV and FDP among the groups .

用体外法观察LW对血凝块的溶解作用；

Lysing effect of LW on blood clot was observed in vitro .

如果开展较大规模的分子流行病学研究，综合各种因素，建议选取血凝块或全血样品，采用盐析法提取DNA。

When molecular epidemiological studies were carried out , it is may be good to collect whole blood or clotted blood sample . About the extraction methods , yield of DNA extracted by Salt is the most ;

大体情况：冲洗后即刻，CIM组原发伤道内坏死组织碎片及血凝块基本被冲净，而RB组仍有较多，BP与冲洗前近似。

The putrescence fragments , blood clots were washed down in CIM , there were a few in RB , and a lot in BP after immediate irrigation .

目的验证小檗碱（BR）抗富含血小板血浆凝块收缩对两种纤溶酶原激活物引发的溶栓作用的协助。

AIM To verify the assisting effect of berberine ( BR ) of its antiplatelet rich plasma clot retraction effect on different plasminogen activator induced thrombolyses .

SPK提取液在体外有明显的抗凝血和直接溶解血凝块的作用。

The SPK extract solution holds obvious effects of anticoagulation and thrombolysis in vitro .

方法：采用同种大鼠无菌自然干燥血凝块颈总动脉注射及跳台反射第1次跳下潜伏期为筛选指标，成功地建立了MID大鼠模型。

Methods : MID rat models were established by injecting sterile dry blood clots into common carotid artery and screening by the first jumping off latency of diving platform reflex .

分别加入高、中、低剂量的DFE溶液、生理盐水和UK溶液后，37℃孵育16小时，比较反应前后血凝块重量的变化。

High , middle and low dose DFE solution , UK solution and physiology saline were added in blood clots , then the clots were incubated at37 ℃ for16hours .

方法：采用Giemsa染色和MTT检测法，经过电镜扫描，分段对比观察，研究3T3细胞在血纤维蛋白凝块上的生长情况。

METHOD : Growth of the cells on blood fibrin clot was studied by phase-contrast , scanning and transmission electron microscopy and by Giemsa stain and MTT assay .

形成血凝块的主要因素，要不然称为DVT或深度静脉血栓形成，并不是旅途时间太长。

One of the main risk factors for a blood clot , otherwise known as DVT or deep venous thrombosis , is not moving around for long periods of time .

方法通过颈外静脉注入125I标记人纤维蛋白原的大鼠加热血凝块，建立不同时间大鼠的肺血栓栓塞症（PTE）模型。

Methods125I-labeled human fibrinogen heated blood clots were prepared in vitro and injected into the external jugular vein to establish rat models of pulmonary thromboembolism ( PTE ) .

手术时间（79.9±33.1）min，术中清除血凝块及不凝血（567.5±177.8）ml，术后24~48h拔除胸腔闭式引流管，术后引流量（220±45.6）ml。

The time of operation was 79.9 ± 33.1 min. The amount of blood clots and noncondensing blood cleared was 567.5 ± 177.8 ml. The closed thoracic drainage tube was removed at 24 ~ 48 h postoperatively , with a drainage volume of 220 ± 45.6 ml.

扩散盒血浆凝块培养中E-CFU-D性质的分析

Analysis of the properties of e-cfu-d by in vivo culture in plasma clot diffusion chamber

本研究从血凝块中提取的DNA为模板用于金属基质蛋白酶3（mmp3）基因PCR扩增获得满意效果，凝胶电泳背景清晰，无非特异扩增带。

In this study , extracting DNA from the blood clot and using it as the template for metal matrix 3 ( mmp3 ) gene PCR amplification can get satisfied results with a clear gel background and no more than specific amplification .

前言：分析了超高温（UHT）个乳常见的缺陷，如脂肪上浮、凝块、变味、酸包、涨包、苦包等产生的原因，同时针对各种缺陷探讨了解决问题的办法。

The common defects of UHT milk such as : fat separation , coagulation , sour package , blown package and bitter package were investigated , meanwhilesolutions in regarding to those issues were discussed .