亲和色谱

用染料配体亲和色谱纯化DNA聚合酶α

Purification of DNA Polymerase α by Dye Ligand Affinity Chromatography

伴刀豆球蛋白亲和色谱柱的制备及其在糖蛋白核糖核酸酶B结构分析中的应用

Preparation of a Concanavalin A Immobilized Affinity Column and Its Application in the Structural Analysis of Ribonuclease B

小鼠血清IgG的蛋白G灌注亲和色谱行为的研究

Chromatographic Behavior of Mouse Serum IgG in Protein G Perfusion Affinity Chromatography

腹水mAb经ProteinA亲和色谱柱纯化后，纯度达92%以上。

The purity of mAb was over 92 % after protein A affinity chromatography .

一种新型IgM高效膜亲和色谱柱的制备

Preparation of a novel high performance membrane chromatographic IgM column

一种肝癌特异性GGT的DSA亲和色谱检测方法

Hepatoma-Specific Glutamyltranspeptidase Detected by DSA Affinity Chromatography Method

结论：以等度洗脱、pH梯度与盐梯度洗脱串接组合的阴、阳离子交换示差组合色谱是一种较好的组合纯化的方式，达到亲和色谱的纯化效果。

Conclusion Differential combination chromatography of anion and cation exchange can get good result which can be got under the affinity chromatography conditions in the purification of proteins .

特异性鸡蛋黄抗体的毒理学安全性研究利用鸡蛋特异性抗体IgY的免疫亲和色谱

Toxicological Assessment on Prepared IgY Safety Immunoaffinity Purification of Specific Immunoglobulin from Egg Yolk

抗奶牛乳腺炎特异性IgY的制备及活性研究利用鸡蛋特异性抗体IgY的免疫亲和色谱

Preparation and Activity Research of Egg Yolk Antibody ( IgY ) Specific for Dairy Cow Mastitis

制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽（GSH）。

Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione ( GSH ) using chitosan as substrate .

ColiBL21（DE3）表达菌株，利用IPTG诱导表达，并用亲和色谱纯化COMT。

Coli BL21 ( DE 3 ), then induced by IPTG , and purified by affinity chromatography .

结果表明，对于小鼠血清中的IgG灌注亲和色谱的分离，由于解离速率影响到传质过程，使之在高流速下的分离受到一定的限制。

The results indicated that the association / dissociation kinetics between IgG and protein G affinity packings became the rate-limiting step in perfusion affinity chromatographic separation process .

抗rhIL-2单克隆抗体高效亲和色谱填料的制备及其应用

Preparation of HPAC and purification of rhIL-2

ColiBL21（DE3）中胞内诱导表达P9ZFD蛋白，组氨酸亲和色谱法纯化P9ZFD蛋白并免疫Balb/c小鼠制备其多克隆抗体。

Coli BL21 ( DE3 ) and purified by histidine affinity chromatography . P9-ZFD antibody was prepared through immunizing Balb / c rats with purified P9-ZFD protein .

结论Glutathionesepharose4B亲和色谱纯化重组汉滩病毒核蛋白是一种较为有效的方法。

Conclusion Purification of nuclear protein of Hantaan virus with Glutathione Sepharose 4B affinity chromatography is an effective method .

方法采用优球蛋白沉淀、离子交换色谱、（NH4）2SO4沉淀和亲和色谱法进行纯化。

Methods A combination of euglobulin precipitation , ion-exchange chromatography , ( NH4 ) 2SO4 precipitation and affinity chromatography was used in the process of purification .

单克隆抗体SZ-95亲和色谱纯化人血小板第4因子

Purification of human platelet factor 4 by monoclonal antibody ( SZ-95 ) affinity chromatography

采用高效亲和色谱技术（HPAC）对中药成分与人血清白蛋白（HSA）的相互作用进行了研究。

The interaction of two Chinese medicinal ingredients and human serum albumin ( HSA ) has been investigated by high performance affinity chromatography ( HPAC ) .

主要是模拟ProteinA与IgG间的相互作用，将计算机辅助药物分子设计与异位合成方法相结合，合成ProteinA仿生分子，并利用亲和色谱技术评价其选择性及其与IgG等蛋白间的相互作用力。

In this paper , a new technology for synthesizing biomimetic ligands will be studied . At the base of simulating the interaction between protein A and IgG , a novel biomimetic ligand of protein A was screened and synthesized by the combination of molecular docking and exsitu-synthesizing .

最后，利用亲和色谱实验验证候选多肽的亲和性，获得了与IgG具有高亲和性的七肽YFDWRWE。

Finally , affinity chromatography was carried out to verify the affinity of candidate peptides , and a high affinity heptapeptide ( YFDWRWE ) of IgG was selected .

用CZE分析抗独特型抗羟甲芬太尼抗体（IgG），表明用ProteinA－Sepharose亲和色谱分离得到部分纯化的IgG，迁移时间不同于对照豚鼠的IgG。

The results coincided with SDS-PAGE which exhibited two bands in the plate . The analysis of anti-idiotypic anti-ohmefentanyl antibody by CZE indicated that the sample was puri-fied partially by Protein A-Sepharose affinity chromatography and distinguished from unimmunized guinea pig antibody .

在利用基因工程技术生产生物医药蛋白的过程中，通常在目标蛋白质尾部添加一段（His）6标签，以便于利用固定化金属离子亲和色谱（IMAC）对目标蛋白质进行分离纯化。

In the production process of biopharmaceutical proteins , most of target proteins were often expressed with a 6 × Histag using genetic engineering techniques , which is convenient to be purified by immobilized metal ion affinity chromatography ( IMAC ) .

然后用蛋白折叠液相色谱法（PFLC），包括阴离子交换色谱、金属离子亲和色谱和疏水色谱对rhG-CSF进行复性，提高了rhG-CSF在不同色谱模式下的质量回收率。

Then , the solubilized rhG-CSF at alkaline solution was proceeded by protein folding liquid chromatography ( PFLC ), including ion-exchange chromatography ( TEC ), immobilized metal chelate affinity chromatography ( IMAC ), hydrophobic interaction chromatography ( HIC ), respectively .

金属螯合亲和色谱中固定金属与蛋白质的作用

Interaction between Immobilized Metal and Protein in Metal Chelate Affinity Chromatography

金属螯合亲和色谱介质的合成及其色谱特性研究

Synthesis of Metal Chelate Affinity Chromatographic Medium and Studies of Chromatographic Characteristics

染料膜亲和色谱法中膜堆的制备及应用

Preparation and Application of the Cartridges . of Dye Membrane Affinity Chromatography

膜亲和色谱的现状、发展和应用

Current Situation , Development and Applications of Membrane Affinity Chromatography

亲和色谱分离纯化低分子肝素

Purification of the low molecular weight heparin by affinity chromatography

氯霉素标准品在免疫亲和色谱柱中的添加回收率为99.8%。

The recovery ratio of CAP in immunoaffinity column is 99.8 % .

免疫亲和色谱-气相色谱/质谱法（IAC-GC/MS）检测猪肝中的沙丁胺醇与克伦特罗

Immunoaffinity Chromatography-GC / MS for Determination of Salbutamol and Clenbuterol in Swine Liver