The pcGH gene was cloned into pET-22b ( + ) for intracellular expression . Recombinant plasmid ( pG2 ) were transformed into E.
通过PCR引物设计，在pcGH基因两端引入两个酶切位点，将其插入到大肠杆菌表达载体pET-22b（+）中，构建胞内表达质粒pG2。
pg2