pc12

Objective To observe the effects of electromagnetic acute irradiation on PC12 cells .

目的探讨电磁辐射急性辐照对PC12细胞的影响。

RESULTS : ① PC12 cell of normally cultured were round shape .

结果：①正常培养的PC12细胞为圆形，呈簇状生长。

Method PC12 cell was exposed to H2O2 in vitro to establish oxidative injury model .

方法体外细胞培养，H2O2处理PC12细胞复制氧化损伤模型。

PC12 cell line was purchased form the Cell Center of Chinese Academy of Medical Sciences .

PC12细胞株购自中国医学科学院细胞中心。

Extracellular glutamate levels of PC12 cells were detected by HPLC .

采用HPLC法测定细胞外液中谷氨酸的浓度。

PC12 cells were treated with H2O2 and glutamate respectively as oxidative stress damage cell model .

本研究采用过氧化氢和谷氨酸纳作用于PC12细胞，成功的制作了氧化应激损伤的细胞模型。

The morphology of PC12 cells was observed by phase-contrast microscopy .

以倒置相差显微镜观察PC12细胞神经突起的变化；

The culture supernatant could stimulate the neurite outgrowth of PC12 cells .

阳性克隆培养上清能促进PC12细胞突起生长。

The neuron differentiation function of different truncated mutants was observed by PC12 transient transfection assay .

通过瞬时转染PC12细胞实验研究不同截断突变体的促神经细胞分化功能。

Rotenone was used on NGF-differentiated PC12 cells to induce apoptosis .

以NGF诱导后的PC12细胞作为细胞模型，采用鱼藤酮诱导细胞凋亡。

Objective To investigate the effect of propofol on cytotoxicity of bupivacaine on PC12 cells and its possible mechanism .

目的探讨异丙酚对布比卡因诱导的PC12细胞毒性的作用及其可能机制。

These results demonstrate that the morphologically differentiated neuronal PC12 cells still retain the ability to divide .

这些结果说明，形态发生分化的神经元样PC12细胞仍有分裂能力。

This is the first report that neuronal PC12 cells as well as their neurites can divide .

本研究首次报道神经元样PC12细胞及其突起能发生分裂。

Methods An ischemic model was developed in PC12 cell line with treatment of oxygen glucose deprivation ( OGD ) .

方法采用氧葡萄糖剥夺（OGD）方法在PC12细胞上建立缺血模型。

Aim To investigate the effects of lipophilic fraction of Ganoderma lucidum on PC12 differentiation .

目的研究灵芝酯溶性提取物对PC12细胞分化的影响。

The protective effects of melatonin on injury of pheochromocytoma ( PC12 ) cells induced by A β 25-35

褪黑素对Aβ（25-35）诱发PC12细胞损伤的保护作用

Conclusion : The neurons differentiated by NGF in cultured PC12 cells can divide in amitotic way .

结论：NGF诱导PC12细胞分化的神经细胞能以无丝分裂方式增殖；

The well growth and the good biological activities of the retrieved PC12 cells were proved using the MTT and Trypan blue test .

回收微囊化PC12细胞再培养生长良好，MTT法和台盼兰法检测显示细胞具有生物活性。

Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma ( PC12 ) cells

新霉素抑制PC12细胞烟碱受体介导的反应

The phase contrast microscope was used on 84 h after transfection to observe the change of the morphology of cultured PC12 ;

转染后84h用相差显微镜观察PC12细胞形态的变化；

This is a study on injurious effects of cellular spermidine to PC12 cells irradiated by enriched uranium .

研究PC12细胞内精脒含量在浓缩铀诱导细胞凋亡中的作用。

The fluorescence microscope technique was employed to observe the effect of BS on apoptosis of PC12 cells induced by Glu .

采用荧光显微镜技术观察BS对谷氨酸所诱导PC12细胞凋亡的影响；

Conclusions : Estradiol may has a neuroprotective role in PC12 cells injury induced by6-OHDA through an antioxidant mechanism .

结论：雌激素可能是通过非受体依赖的抗氧化机制对6-OHDA引起的PC12细胞损伤起保护作用。

Conclusion There are specifically oxidized proteins in PC12 cells , which can be used as reliable frames of reference under oxidative damage conditions .

结论处于基础状态的PC12细胞存在蛋白质的氧化修饰，可以为病理条件下的氧化损伤研究提供可靠的参照系统。

Furthermore , the lower anti-A β antibodies in the serum from AD patients and healthy elderly individuals can protect the PC12 cells .

而健康老人的血清仍能表现出较强的PC12细胞保护作用。

Results ; Reserpine , an inhibitor of VMAT , itself had no poisonous effect on PC12 cells .

结果：单独加入VMAT抑制剂利血平对PC12细胞无毒性作用；

The retrieved encapsulated PC12 cells were recultured and their activities were tested by MTT and Trypan blue test .

对移植的微囊化PC12细胞回收后再培养，以MTT和台盼兰检测细胞活性。

Methods PC12 cell was irradiated with DMEM / F12 agent of enriched uranium , and the internal exposure doses were calculated .

方法在PC12细胞中加入浓缩铀DMEM/F12工作液，计算PC12细胞的内照射吸收剂量。

Results : PC12 cells showed a decreased viability in response to the treatment of glucose deprivation , and most cells were necrosis or apoptosis after 48 hours .

结果：PC12细胞在无糖培养条件下，细胞活力逐渐下降，48小时后，大部分细胞死亡，部分细胞为凋亡。

Effect of cleistocalyx operculatus extracts on intracellular and extracellular oxygen-derived free radicals in PC12 nerve cells induced by oxidative injury .

水翁花提取物对氧化损伤的PC12神经细胞内、细胞外氧自由基水平的影响。