ad5

Transfection Efficiency of Adenoviral Vector AD5 / F35 to Malignant Hematopoietic Cells of Different Origins

AD5/F35腺病毒载体对不同来源造血系统恶性细胞转染效率的研究

Ad5 β gal was applied to transfect the adipose tissue-derived multipotent stem cells .

用Ad5βgal转染脂肪组织来源的多能干细胞。

Results The identification of PCR and enzyme cutting showed that the construction of the recombinant Ad5 / F35-hBMP-7 plasmid could be confirmed .

结果PCR、酶切鉴定表明rAd5/F35-hBMP-7质粒构建正确。

Methods Recombinant adenovirus Ad5 / VP4 was constructed by homologous recombination , proliferated in 293 cells and purified .

方法在293细胞上扩增表达猴轮状病毒SA11株VP4抗原的重组腺病毒并纯化。

The adenovirus subgenus C serotypes 5 ( Ad5 ) vectors which have been used to transduce epithelial cells have very limited ability to infect hematopoietic cells .

人C组5型腺病毒（Ad5）载体能够有效感染上皮来源的细胞，但对造血细胞的感染效率很低，限制了其在造血调控基础研究以及血液病基因治疗中的应用。

The AD5 / F35 vector is preferable to AD5 vector in respect of infection ability and offers good prospects of application in gene therapy for myeloid leukemia cells as target cells .

AD5/F35载体优于常用的5型腺病毒载体，在以髓系细胞为靶细胞的基因治疗中有着较好的应用前景。

Conclusion The successful construction of ad5 / CMV-TGF - β 1 production in our experiment may lay foundation for the orthopaedic gene therapy such as in the treatment of the defect of bone , muscle tissue .

结论重组缺陷型腺病毒载体Ad5/CMV-TGF-β1的构建成功，为下一步进行基因疗法治疗骨骼、肌肉组织缺损提供基础。

Objective To study the effect of cell mediated immunity of a novel recombinant adenovirus ( Ad5 / 35 ) vector-based HIV vaccine ( Ad5 / 35-HIV ) prepared by Yokohama City University .

目的探讨新型重组腺病毒5/35载体HIV疫苗（Ad5/35-HIV）刺激小鼠产生IFN-γ分泌型T细胞的效果。

Conclusion The recombinant adenovirus Ad5 / VP4 expressing rotavirus VP4 antigen induced specific IgA and IgG in milk and showed a protective rate of 100 % to the neonatal mice challenged with rotavirus .

结论表达轮状病毒VP4抗原的重组腺病毒经滴鼻免疫母鼠后，能在乳汁中产生特异性的IgA和IgG抗体，并能保护新生小鼠对抗轮状病毒的攻击，保护率达100%。

The recombinant adenovirus was analyzed with PCR finally . Result The ad5 / CMV-TGF - β 1 obtained with homologous recombination was stable and at high viral titer ( 5 ~ 10 × 107pfu / ml ) .

结果采用同源重组的方法获得的重组复制缺陷型腺病毒载体Ad5/CMV-TGF-β1稳定、滴度高（5～10×107pfu/ml）。

Methods Replication defective adenovirus type 5 ( Ad5 ) vector encoding the rat gax gene ( AdCMV gax ) was constructed via site specific recombination and amplified in 293 cells . The resulting adenovirus was purified for a high viral titer .

方法采用位置特异性重组方法构建携带大鼠gax基因表达序列的复制缺陷型5型腺病毒载体（AdCMVgax），经293细胞扩增，纯化制备高滴度病毒转染液；

Methods The cells from the embryonic rat cortices were mechanically dissociated and the N2 medium was adopted to culture and expand the cells . Then the cells were identified by immunocytochemistry method , and the Ad5 β gal vector was applied to test their capacity of expressing foreign gene .

方法从胚胎大鼠脑皮质中机械分离细胞，应用N2培养基进行培养和扩增，采用免疫组织化学方法进行鉴定，应用Ad5βgal重组腺病毒载体检测神经干细胞表达外源基因的能力。