RecA

Construction and Expression of PEA recA Fusion Gene of P aeruginosa

绿脓杆菌外毒素ArecA基因的融合及融合蛋白的表达

It is concluded from the experimental results that the recA gene functions at the level of DNA replication .

实验结果说明recA基因在DNA复制过程中起作用。

Cloning and Sequential Analysis of Mycobacterium bovis RecA Gene

牛分枝杆菌RecA基因的克隆与序列分析

Coli chromosome DNA as the template , an intact recA gene was obtained by PCR .

coli染色体DNA为模板进行PCR扩增，获得完整的recA基因。

New genes were obtained . The works reported here throw some light on the understanding of the function of the recA gene .

本文的工作为recA基因的功能提供了新的认识。

In Escherichia coli most recombinations depend on intracellular RecA protein .

大肠杆菌的同源重组依靠内源性的RecA蛋白。

Those with the plasmid integrated close to terC were recA dependent even on minimal medium .

整合在rerC附近的在不丰富的培养基上也依赖于recA基因。

Catalyst of D.Radiodurans LexA Protein and Its Induced Catalysis at Alkaline pH Activated by RecA

RecA激活的抗辐射菌LexA蛋白降解及其在碱性条件下的诱导降解

The research includes the construction of wild rice transformation-competent genomic library and the RecA protein mediated biotinylated probes enrichment library .

它包括构建野生稻可转化基因组文库，利用RecA重组蛋白介导生物素标记的探针对文库进行磁珠富集，构建候选抗性基因富集文库。

In a previous paper we reported that the replication of chromosome initiated by the integrated F ' plasmid was recA gene dependent .

前文[2]报道了F质粒整合后所发动的染色体复制依赖于recA基因。

To date , little is known about the biochemical mechanism controlling the expression of D. radiodurans RecA and PprA .

到目前为止，有关耐辐射球菌RecA和PprA的诱导表达分子机制还不清楚。

Experimental Study of Piezoelectric Gene Sensor by PNA and the System of Biology Magnified of " RecA Protein-complementary Single Strand DNA " Probe

肽核酸和RecA蛋白&互补单链DNA探针生物信号放大系统用于压电基因传感器的实验研究

The concentrations of RecA protein and ATP γ S were optimized respectively . And the frequency shift and reaction time were observed under the optimum conditions .

首先分别优化RecA蛋白及ATPγS的浓度，再观察最佳条件下传感器检测系统的频率下降值和反应时间。

The research comprehends the candidate resistance gene cloning strategies , utilization the ability of RecA enrichment library and the transformation-competent genomic library technology .

本研究集成了候选抗性基因克隆技术和磁珠富集文库技术以及可转化基因组文库技术，提出了基于候选抗性基因富集文库克隆野生稻新抗性基因的策略。

Replication Characteristics of Plasmids . and Their Dependence of recA Gene for the Initiation of Escherichia colt Chromosome Replication in the Integrated State

质粒的复制特性和整合后在发动大肠杆菌染色体复制中对recA基因的依赖性的研究

Dependence of the recA Gene for the Replication of the Bacterial Chromosome Initiated by the Integrated F ' Plasmid in Escherichia colt

大肠杆菌中整合的F′质粒带动细菌染色体复制需要recA基因

Those with the plasmid integrated between oriC and terC were recA dependent on rich medium but independent on minimal medium ;

整合在oriC近旁的Sin菌株不依赖于recA基因；整合在oriC和terC中间的只在丰富培养基上是依赖的；

Results The frequency was reduce obviously when the concentration of RecA protein was 3mg / ml and that of ATP γ S was 12.5 μ M.

结果当RecA蛋白浓度为3mg/ml时，ATPγS浓度为12.5μM，所引起的频率变化最显著。

Recently , polyamines have been shown to mediate the production of RecA , with the the LexA control the SOS response regulator .

最近，多胺已被证明调解RecA的生产，配合LexA控制SOS反应调节子。

LexA protein is the main SOS response modifiers , until the DNA damage activates RecA protein with function as a transcriptional repressor .

LexA蛋白是主要的SOS反应调节剂，作为转录阻遏蛋白行驶功能直至DNA损伤激活RecA。

Regulatory Proteins of RecA in Deinococcus Radiodurans

耐辐射球菌中RecA的调控蛋白研究

The biosynthesis of DNA and protein of the Sin recA + and Sin recA-strains at different tern peratures were compared .

比较了SinrecA~+和SinrecA~-菌株在不同温度中的DNA、蛋白质的生物合成情况。

Conclusion The sensitivity of piezoelectricity gene sensor array is improved and the time of procedure is decreased significantly by using the probe of RecA protein-complementary single strand DNA in reaction system .

结论在反应体系中加入RecA蛋白-互补单链DNA探针，能有效地提高压电基因传感器的灵敏度，明显缩短反应体系时间。

Objective To improve the detection sensitivity of peptide nucleic acid ( PNA ) piezoelectric gene sensor by introducing RecA protein-complementary single strand DNA probe complex as new style biological signal amplification system .

目的在构建好的肽核酸压电基因传感器检测系统上引入RecA蛋白互补单链DNA探针复合体作为新型生物信号放大系统，提高传感器的检测灵敏度。

We have reported the dependence of recA gene for the replication of chromosome of E. colt initiated by the F ' plasmid but not the F plasmid .

我们曾报道整合的F′质粒所发动的大肠杆菌染色体复制依赖于recA基因，而整合的F质粒则不。

Methods A semi-quantitative RT-PCR two-step method was developed to determine LDH gene against the housekeeping gene recA . Gene expression level of LDH was assayed by gel documentation system and QUANTITY ONES software .

方法应用半定量RT-PCR两步法和凝胶成像系统定量软件分析，分别对20株来自不同基因型的LDH进行基因表达水平差异的评价和比较。

It also indicates that the RecA protein could interact with the λ CI repressor without any activating signals , or by the activation of other signals , not only the ssDNA .

这也表明RecA蛋白可能在其它激活信号的激活下也可以与λCI阻遏物发生作用，而不是仅仅受ssDNA的激活。

By RecA protein mediated homologous recombination method , we successfully deleted the HS-40 fragment from a bacterial artificial chromosome containing complete human α - globin gene cluster screened by our lab.

采用RecA蛋白介导的同源重组的方法，对本实验室筛选出的包含完整人α类珠蛋白基因簇的细菌人工染色体（BAC）DNA进行删除修饰。

The results indicated that the recA gene showed a remarkable physiological function in the λ lysogenic bacteria . It could induce the λ prophage to enter the lysis cycle from lysogenic state .

结果表明recA基因在溶原菌中表现出明显的生理功能，能使λ原噬菌体从溶原状态进入裂解循环。

The reported experimental results tend to suggest that the site of integration is of primary importance in the dependence vs independence of recA gene for the replication of the chromosome initiated by the integrated plasmid .

实验结果说明，质粒的整合位置是决定由整合质粒所发动的染色体复制对recA基因的依赖性的主要因素。