ENA of the virus was extracted by phenol-chloroform and precipitated by ethanol . cDNA probe was labeled by 33P according to nick translation method . Hybridization was carrying on under 42 ℃ .
采用酚-氯仿提取法和乙醇沉淀法提取标本中的RNA，cDNA探针以缺口翻译法标记~（32）P同位素，在42℃条件下进行cDNA-RNA分子杂交。
33p