32a

Response of Fertility of ⅱ - 32A to Temperature and Daylength and Its Sensitive Growth Stages

Ⅱ-32A育性光温反应及敏感生育期的研究

Purification of Rice CMS Line ⅱ - 32A

水稻不育系Ⅱ&32A连续提纯及其纯系表现

32A large herd of pigs was feeding there on the hillside .

那里有一大群猪在山上吃食。

32A crowd was sitting around him , and they told him ," Your mother and brothers are outside looking for you . "

有许多人在耶稣周围坐着，他们就告诉他说：“看哪，你母亲和你弟兄在外边找你。”

ⅱ You 845 (ⅱ - 32A × Wenhui 845 ) is a new hybrid rice combination developed by Wenzhou Academy of Agricultural Sciences .

Ⅱ优845是温州市农科院用Ⅱ-32A与自育恢复系温恢845配组育成的杂交水稻新组合。

Baifeng A is similar to ⅱ - 32A in male sterility , flowering habits , major agronomic characters , rice grain quality , disease resistance and combining ability .

白丰A的不育性、开花习性、主要农艺性状、稻米品质性状、抗病性、配合力均与Ⅱ-32A相似。

The restoration of fertility of Fujian ⅱ 32A was also enhanced by short daylength , and there existed complimentary effect of temperature and daylength on its fertility .

福建提纯的Ⅱ32A育性恢复还受短光长促进，并存在光温互作效应。

These genes were inserted in plasmid pET 32a and the prokaryotic proteins were expressed under induction of 1 mM IPTG . The recombinant proteins were purified and identified .

目的基因连入pET32a载体，1mMIPTG诱导原核蛋白表达，并对表达的蛋白进行纯化和鉴定。

The SS gene was cloned into the pET 32a and pET 32c expression system respectively Two recombinant plasmids SS N / X and SS N / S were gained correspondingly .

分别将SS基因克隆至pET32a和pET32c表达系统中，得到重组质粒SSN/X和SSN/S。

After that , the genetically engineered bacteria which including pET 32a ( + ) Der f 2 plasmids were induced by IPTG , the expression product was analyzed by SDS PAGE and densitometric scanning .

重组质粒pET32a（+）Derf2转化大肠埃希菌，IPTG诱导表达后进行SDSpage电泳和薄层凝胶扫描定量分析。

Baifeng A , a new rice CMS line with a green-revertible albino leaf color marker , is developed through crossing and successively backcrossing ⅱ - 32A with the leaf color mutants derived from the radiation-induced maintainer line ⅱ - 32B by gamma rays .

白丰A是用60Coγ射线诱变保持系Ⅱ-32B干种子，筛选叶色突变体并与Ⅱ-32A杂交和连续回交转育而成的苗期携带白化叶色标记的不育系。