调控序列

随着ENCODE计划的逐步深入，基因组复杂的散在调控序列，大量的非编码RNA基因以及非编码区域的保守元件浮出水面。

As the ENCODE project is done gradually , complex scattered regulatory sequences of the genomes , a large number of non-coding RNA genes and conservative elements of non-coding regions are found .

植物基因调控序列及其转录起始频率分析

Analysis of Regulatory Sequences and Transcriptional Initial Frequency of Plant Genes

以及构建好的山羊β-酪蛋白基因的调控序列C。

The regulative sequence C of 3 - casein gene had been constructed .

绵羊β-乳球蛋白基因5′端及上游调控序列的PCR扩增的方法学研究

Study on Amplification of BLG 5 ′ - Flanking and Regulatory Sequence by PCR

启动子是DNA序列上的一段重要的基因调控序列，标志着转录起始点的位置，可以用来定位基因。

Promoter is a gene regulatory DNA sequence , which indicates the location of Transport Start Site and can locate gene .

以含有抗生链霉菌酪蛋白酶基因的质粒pIJ702为模板，用PCR方法扩增得到了melC1的信号肽编码序列及其上游的调控序列。

The melC 1 signal peptide-encoding sequence and its upstream sequence originated from S. antibioticus was also amplified by PCR technique .

此外，利用连接介导的染色体步行技术，克隆了全长1768bp的马尾松PS基因上游调控序列。

The upstream sequence of PS gene was cloned by genomic walking based on ligation-mediated PCR method .

人CD34分化抗原转录调控序列的克隆与调控表达

Cloning and Regulating Expression of Human CD34 TRS

乳清酸蛋白基因调控序列的鉴定及转基因小鼠乳腺表达G-CSF载体的构建

WAP Gene Identification and Construction of Vectors Expressing Human G CSF

蛋白激酶C活性下降对Ha-ras基因表达及其调控序列结合蛋白的影响

The effect of inhibition of protein kinase C activity on the Ha-ras oncogene expression and its binding protein

位于该调控序列内的ALB高效表达。

The exogenous gene ALB was highly expressed in the regulating sequence .

SPM法获取基因上游调控序列

Getting upstream gene regulatory area by SPM

获得含NBS保守域的EST，进一步用RACE技术和TAILPCR技术获得其中1个EST的全长基因序列，并获得GHNBS基因的5′调控序列。

The full length of one EST and its 5 ′ flanking sequence was acquired by RACE and TAIL PCR methods .

目的研究mtr基因调控序列突变与曲拉通耐受的关系。

Objective To study the relationship between triton resistance and mtr controlling gene region mutation .

研究表明：在洋葱表皮细胞中，160bp及更长的上游调控序列均能启动GUS基因的表达；

In onion epidermal cells , the 160 bp fragment and longer ones were functional for directing GUS expression .

大鼠20αHSD基因调控序列的克隆与检测

Cloning and assay of regulating sequence of the rat 20 α hsd gene

北京奶山羊β乳球蛋白基因（βlg）的克隆及其调控序列的分析

Cloning of Beijing Milk Goat β - lactoglobulin Gene (β lg ) and Analyzing Its Control Regions

山羊BLG基因5′调控序列克隆及其调控GFP基因细胞的表达

Cloning of 5 flanking sequence of goat BLG and regulating the expression of GFP in cell

目的：构建含人乳癌DF3/MUC1启动子转录调控序列和白喉毒素A链（DTA）基因的重组表达载体。

Aim : To construct recombinant expression vector containing transcriptional regulation sequence of human breast cancer DF3 / MUC1 promoter and diphtheria toxin A-chain gene .

另外，Egr-1基因调控序列具有辐射可诱导特性，这一特点使其有了潜在的临床和基因工程生产应用前景。

Additionally , the radiation-inducible property of the Egr-1 gene regulating sequence confers potential clinical and genetic engineering sues .

研究MADS-box基因的调控序列可以进一步揭示影响基因时空表达的内外因素。

Studies on the regulatory sequence of MADS-box genes will reveal spatial and temporal control of gene expression .

牛BLG基因5′调控序列控制GFP基因在原代乳腺上皮细胞中的表达

Expression of GFP Report Gene under the Control of Bovine BLG Gene 5 ′ Regulatory Sequence in Primary Mammary Epithelial Cells

IRS-1基因5′调控序列CAG富含区存在以三核苷酸为单位的多种插入／缺失变异。

There were deletion / insertion variations as trinucleotide repeat sequence in the CAG - rich region of IRS-1 5 ' flanking region .

应用RT-PCR方法检测PRL基因的表达，同时克隆PRL基因5'上游调控序列-1206~-1966bp片段，采用DNA直接测序方法检测该序列的突变情况。

Reverse transcription-polymerase chain reaction was employed to evaluate the expression of PRL mRNA in pituitary glands , and DNA sequencing was used to analyze the mutation in regulatory sequence of PRL gene .

方法提取新生小牛肝组织的DNA，应用PCR分段扩增牛α-S1酪蛋白基因调控序列，经琼脂糖凝胶电泳鉴定后测序，再将各基因片段连接成完整的序列。

Methods Extract DNA from the liver tissue of newborn calf for fractional amplification of bovine α - S1 casein gene regulating sequence by PCR . Identify the amplified product by agarose gel electrophoresis and sequencing , then ligate the fragments into a intact one .

以羊的β-乳球蛋白基因（BLG）作为调控序列，构建了瑞替普酶的乳腺表达载体BrPA。

The rPA fragment was inserted into mammary gland expression vector with β - lactoglobulin gene as a regulation sequence .

结果：构建了含人乳癌DF3/MUC1启动子转录调控序列和DTA的表达载体，酶切和测序结果与预期完全相符。

Results : The expression vector containing transcriptional regulation sequence of human breast cancer DF3 / MUC1 promoter and diphtheria toxin A-chain gene had been successfully constructed and identified by restriction endonuclease and sequencing .

以GUS为报告基因，利用该启动子构建表达载体，农杆菌介导法转化番茄，获得了转基因番茄植株，研究该调控序列的组织表达特异性。

To investigate the tissue expression pattern of the cloned regulatory sequence . an expression vector containing this sequence fused with GUS was constructed for transformation into tomato by using agrobacterium-mediated method .

目的探讨白念珠菌耐药基因CDR1基因上游调控序列对氟康唑耐药的影响。

Objective To appraise the relationship between fluconazole resistance and expression of the CDR1 gene upstream regulation sequence .

目的观察17-β-雌二醇（E2）诱发大鼠原位和移植于肾囊的异体垂体形成催乳素瘤的过程及原位和移植垂体催乳素（PRL）基因调控序列突变的差异性。

Objective To analyze different mutations in regulatory sequence of prolactin ( PRL ) gene during the formation of 17 beta-estradiol ( E_ 2 ) - induced prolactinoma in eutopic and ectopic pituitary of rats .