生物素化

这里，我们通过膜表面生物素化的方法，研究CPE对海马神经元中内源TrkB受体的活性依赖的上膜的调控。

Here , through the surface biotinylation methods , we studies of the effect of CPE on endogenous TrkB receptor activity-dependent surface insertion in hippocampal neurons .

通过生物素化标记分析细胞膜亚蛋白质组

Analysis of Cell Membrane Subproteome by Biotinylation of Proteins

用化学方法制备生物素化λDNA探针。

The biotinylated λ DNA probe was prepared by a chemical method .

生物素化HPV16DNA探针与人宫颈癌组织DNA分子杂交的研究

Study on molecular hybridization with biotin-labelled HPV 16 DNA probe in human cervical carcinoma

通过选择最佳的链霉亲和素浓度和生物素化DNA的浓度，控制了非特异性扩增。

We controlled the nonspecific amplification using the optimal concentration of DNA label and streptavidin .

应用PCR技术合成生物素化蓝舌病病毒核酸探针的研究

Synthesis of Biotinylated Bluetongue Viral RNA Probe by PCR

方法利用亲和素作为桥梁连接生物素化DNA和抗体，形成抗体亲和素DNA偶联物。

Methods Biotinylated DNA and antibody were coupled with avidin , thus an antibody avidin DNA conjugate was prepared .

生物素化羊抗兔IgG（武汉博士德生物技术公司）；

Biotinylated goat anti-rabbit IgG ( Boster Bioengineering Co. , Ltd. , Wuhan );

生物素化ATP硫酸化酶的表达、固定化与应用

Expression , Immobilization and Application of Biotinylated ATP Sulfurylase

目的：可溶性HLAA2抗原肽复合物的体外折叠复性与生物素化。

AIM : To refold and biotinylate HLA A2 peptide complex in vitro .

HCV核心抗原N-端片段生物素化表达质粒的构建及表达

Construction and Expression of Biotinylated Expression Vector for Amino Acid Fragment at N-terminus of HCV Core Antigen

生物素化HDVcDNA探针的制备及其在血清斑点杂交中的应用

The preparation of biotin-labeled HDV cDNA probe and its application to spot hybridization

方法应用17种生物素化的凝集素，采用ABC方法检测了30例小汗腺良恶性肿瘤细胞表面凝集素受体的表达。

Methods The expression of seventeen kinds of lectin-receptors in thirty cases of eccrine gland tumors were detected by ABC method .

洗去细胞，加生物素化抗小鼠IgG以使生物素分子固相比。

Then the cells were removed by washing , and biotinyl anti-mouse IgG was added to introduce biotin molecules onto solid phase .

洗涤后，加亲和素-生物素化HRP复合物（ABC），继加底物溶液。

After washing , avidin-biotinyl HEP complex ( ABC ) was added , followed by the addition of substrate solution .

本实验对生物素化补骨脂素标记核酸的条件和效果进行了研究，发现该试剂不仅能标记双链DNA、单链DNA，更重要的是可以直接标记RNA及寡核苷酸。

We have systematically studied the relations between the probe labelling effects and conditions , and found that it not only can label dsDNA , ssDNA , but also RNA and oligonucleotide .

方法：取76例不同类型突出腰椎间盘组织标本用生物素化羊抗人IgG抗体进行免疫组化染色。

Methods : Seventy six specimens of various herniated disc tissue were immunostained with anti human IgG , using the avidin biotin peroxidase complex method .

方法将生物素化等位基因特异性寡核苷酸固定于生物芯片上长链烷烃硫醇自组装单层已连接链霉亲和素（SA）的部位。

Methods Allele specific biotinylated oligonucleotides were immobilized onto defined areas of streptavidin tethered self assembled monolayers of long chain alkane thiols on biochips .

方法用游离的亲合素将生物素化的二抗和生物素化的腺病毒六邻体基因重组质粒DNA偶联起来，通过PCR扩增标记的DNA检测弓形虫循环抗原，建立了免疫-PCR检测方法；

Methods Free streptavidin was used to attach the biotinylated second antibody to the Bio-AdAT DNA , through amplication of the DNA lable , Toxoplasma circulating antigens was detected , thus Immuno-PCR assay was established ;

利用特异单抗（W6/32和兔抗人β2m抗体）及链霉亲合素进行ELISA和westernblot，检测稀释复性和生物素化的折叠产物。

The refolded and biotinylated products were detected by ELISA and Western blot with monoclonal antibody ( W6 / 32 and rabbit anti-human β _ ( 2 ) m antibody ) and streptavidin .

报道了靶DNA／生物素化λDNA探针／亲合素／Bio－BSA（TG）－DTPA－Eu~（3＋）体系在核酸杂交分析中的应用。

Here we reports a new detection method for λ DNA through the system of target DNA / Bio-DNA probe / Avidin / Bio-BSA ( TG ) - DTPA - Eu3 + .

方法：用链霉亲和素将生物素化的二抗和生物素化的DNA偶联起来，通过PCR扩增标记的DNA检测固定的抗原，建立免疫-PCR技术。

Methods : Free streptavidin was used to attach a biotinylated DNA to the biotinylated second antibody , Antigen which bound with antibodies was detected by amplication of the DNA label , thus Toxoplasma dissoluble antigens Immuno PCR assay was established .

方法：以商品化的抗人BlyS单克隆抗体、生物素化抗人BlyS多克隆抗体，采用双抗夹心法自行建立BlyS血清水平的ELISA检测方法；

Methods : Anti-human BlyS monoclonal antibody and biotinylated anti-human BlyS polyclonal antibody were used to detect serum BlyS levels by ELISA .

研究7种生物素化的凝集素对卵巢Brenner瘤不同分化方向和分化性质瘤细胞的标记特点。

The marker characteristics of different orientations and states of cellular differentiation of ovarian Brenner tumor were studied by using seven biotinylated lectins .

通过生物素化的糖和链霉亲和素键合的量子点结合，我们建立了一种原位观察肿瘤细胞膜表面鼠李糖结合凝集素的方法，并且第一次用这种方法观察到了RBL受体。

Through the combination of biotinylated carbohydrates and streptavidin quantum dots , a method for in situ observation of RBL receptor on cell surface was established .

在H2O2存在下，HRP催化生物素化酪胺大量沉积在目标蛋白周围，通过生物素-亲和素交联将大量荧光探针聚集在微珠表面，实现对目标蛋白的高灵敏检测。

Biotin-labeled tyramine was deposited around the target protein in the presence of HRP and H2O2 , and the fluorescent probes were specifically captured by the biotin-labeled tyramine through biotin-streptavidin linkage , to realize a highly sensitive detection of target protein .

生物素化葡聚糖胺示踪未受损皮质脊髓腹侧束（ventralcorticospinaltract，vCST）的出芽情况。

Biotin dextran amine was used to trace the sprouting of intact ventral corticospinal tract ( vCST ) .

抗体通过单层蛋白A固定到金电极表面，预先处理好的生物素化抗体和辣根过氧化物酶（HRP）标记的亲和素形成的网状复合物作为免疫反应信号的放大探针。

Anti-IgG antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein A. Biotin labeled anti-IgG antibody and HRP-streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody-antigen interaction .

干预：分别给PBN及舌咽和迷走神经内注射四甲基罗丹明（tetramethylrhodamine-dextran，TMR），生物素化葡聚糖胺（biotinylateddextran-amine，BDA）并进行NOS免疫组织化学反应，结合激光扫描共聚焦显微镜观察。

INTERVENTIONS : Tetramethyl rhodamine - dextran ( TMR ) and biotinylated dextran - amine ( BDA ) were injected into the PBN and vago - glossopharyngeal nerves respectively combining with the immunohistochemical method for NOS and the observation with laser confocal scanning microscope .

为了制备聚合量子点，首先需要用生物素化的变性牛血清白蛋白（dBSA）包裹量子点，然后通过生物素与亲和素的相互作用，量子点相互偶联生成聚合量子点。

To prepare the POLY-QDs , CdTe quantum dots were first coated with biotinylated denatured bovine serum albumin ( dBSA ), and then via the effect of the biotin-avidin system , the biotinylated dBSA-coated QDs which had strong fluorescence were conjugated .