激肽释放酶

组织激肽释放酶基因与2型糖尿病关系的研究

Relationship between the polymorphism of the regulatory region of human tissue kallikrein gene and type 2 diabetes mellitus

方法：（1）利用基因重组（geneticrecombination）制备人组织激肽释放酶。

In the present study : ( 1 ) We used the genetic recombination method to produce kallikrein .

方法提取人胰腺组织总RNA，逆转录后PCR扩增激肽释放酶cDNA。回收、补平后插入质粒KS，构建出中间载体KSKK，酶切鉴定后双向测序分析激肽释放酶基因序列。

[ Methods ] Total RNA was extracted from human pancreas and then human tissue kallikrein gene cDNA was amplified by reverse-transcription PCR and cloned to the KS plasmid .

激肽释放酶&激肽系统基因相关SNPs及其单体型与长沙汉族人群脑出血的关系研究

The Study of the Association of SNPs and Haplotype of Genes Involved in the Kallikrein-kinin System with Cerebral Hemorrhage in Changsha Han Chinese

将编码人组织激肽释放酶成熟蛋白的基因片段扩增并分别克隆到原核表达载体pET28（b）及分泌型表达载体pET20（b）中，使其C端融合6×Histag序列。

Human tissue kallikrein gene fragment encoding mature kallikrein was amplified and cloned into pET 28 ( b ) and pET 20 ( b ) plasmid with C terminal 6 × His Tag .

目的：观察实验性脑缺血再灌注损伤后不同时期组织型激肽释放酶活性、缓激肽含量、缓激肽B1、B2受体的动态表达情况，并探讨其在脑缺血病理生理过程中的作用。

Aim : To observe the concentration of bradykinin , the expression of B1 and B2 bradykinin receptors at different time points after cerebral ischemia and reperfusion .

人腺体激肽释放酶基因重组表达载体pPICZαChK2的构建

Construction of recombinant human glandular kallikrein expression vector pPICZ α ChK2

本文主要就激肽释放酶激肽系统（KKS）在心血管领域的研究进展进行综述。

This paper is to review the researches on the kallikrein kinin system in cardiovascular system .

克隆人胰腺组织激肽释放酶基因（hKK），构建融合荧光蛋白基因的真核表达载体，在CHO细胞中表达，为开发激肽释放酶基因工程产品以及开展基因治疗高血压研究奠定了基础。

Molecular cloning and expression of human tissue kallikrein gene fused with EGFP report gene in CHO cells for the development of human kallikrein products and further study in gene therapy .

方法用比色法测定激肽释放酶（TKA）活性，用HOE140和胰激肽释放酶改变激肽系统的功能。

Methods The TKA activation was measured and the function of kinin-system was changed by the antagonist of bradykinin B2 receptor-HOE 140 and kallikrein .

缓激肽是血浆激肽释放酶&激肽系统的基本组成部分，具有缓激肽B1和缓激肽B2两种受体，其中缓激肽B2受体在糖尿病肾病的发生、发展中起着重要作用。

Bradykinin is an essential component of kallikrein kinin system , which have two receptors : bradykinin B1 receptor and bradykinin B2 receptor . The latter receptor plays an important role in diabetic nephropathy .

人组织激肽释放酶（HK）是一种分泌型丝氨酸水解酶，家族成员有15个，由激肽释放酶基因（KLKs）所编码，15个基因均位于染色体19q13.3-13.4，相继排列。

It is know clear that the human tissue kallikreins ( HKs ) are serine proteases , which contain 15 members , encoded by kallikrein gene family ( KLKs ) .

人尿激肽释放酶（SK-827）对兔脑缺血/再灌注损伤的影响

The Effects of Human Urinary Kallidinogenase ( SK-827 ) on Brain Ischemia / Reperfusion Injury in Rabbits

目的探讨重组人组织型激肽释放酶（HK）基因腺相关病毒载体（rAVV/HK）对体外培养的人脐静脉内皮细胞（HUVECs）作用。

Objective To investigate the effect of recombinant aden o-associated virus vector ( AVV ) expressing the human tissue kallikrein ( HK ) ge ne ( rAVV / HK ) on human umbilical vein endothelial cells ( HUVECs ) in vitro .

背景与目的肾素-血管紧张素系统（RAS）和激肽释放酶-激肽系统（KKS）都是由许多大分子物质组成，参与许多生理和病理过程。

BACKGROUND AND OBJECTIVES The renin – angiotensin system ( RAS ) and the kallikrein – kinin system ( KKS ) each encompasses a large number of molecules that participate in many diverse aspects of physiology and pathophysiology .

用放射免疫分析法测定受照大鼠组织激肽释放酶的变化

Detecting the changes of rat tissue kallikrein after irradiation by RIA

人类激肽释放酶基因4、5在卵巢癌中的表达

Expression of Human Kallikrein Gene 4 and 5 in Ovarian Cancer

一种有效分离人尿激肽释放酶和人尿胰蛋白酶抑制剂的方法

An Effective Method for Isolating Human Urinary Kallikrein and Trypsin Inhibitor

目的构建并鉴定人组织激肽释放酶基（h-TKK）基因真核表达质粒。

Objective To construct human tissue kallikrein gene eukaryotic expressing vector .

人组织激肽释放酶成熟蛋白在大肠杆菌中的高效表达

Overexpression of Mature Human Tissue Kallikrein Fusion Protein in E. coli

重组杆状病毒介导的人组织激肽释放酶在昆虫细胞中的表达

Expression of Recombinant Human Tissue Kallikrein Using Baculovirus-Insect Cell Expression System

发色多肽用于前激肽释放酶活性测定的研究

Research on Chromogenic Peptide in Determination of Prekallikrein / kallikrein Activity

人组织激肽释放酶基因真核表达载体的构建与鉴定

Construction and identification of human tissue kallikrein gene eukaryotic expressing vector

转导激肽释放酶基因对高血压大鼠的作用

Effects of gene delivery of human tissue kallikrein into hypertensive hats

激肽释放酶能将前尿激酶活化为双链结构的尿激酶；

Pro urokinase can be activated to double chain urokinase by kallikrein .

激肽释放酶的快速分离纯化新工艺

A new rapid technology for separation and purification of kallikrein

组织激肽释放酶家族、激肽释放酶-激肽原-激肽系统及其重要应用

New human tissue kallikrein gene family kallikrein-kininogen-kinin system and association to disease

人组织激肽释放酶基因在哺乳动物细胞中的表达

Molecular Cloning and Expression of Human Pancreatic Kallikrein Gene in CHO Cell

作者研究了血浆和组织激肽释放酶激活纤溶酶原的机制。

We studied the mechanism of plasma and tissue kallikrein in activating plasminogen .

人尿源性激肽释放酶在家兔和大鼠中的药代动力学

Pharmacokinetics of human urinary kallidinogenase in rabbits and rats