标记基因

目前，正结合进行田间分离纯合和DNA分子鉴定，培育去除选择标记基因的转基因抗虫玉米自交系。

At present , inbred lines of transgenic insect resistant maize with selective marker gene removed are in separating and inbreeding program assisted by DNA marker detection .

裸DNA肌肉注射不同注射方法对标记基因转染效率的影响

The Effect of Different Injection Techniques of Naked DNA on Marker Gene Transfer Expression

利用除草剂抗性基因bar作为标记基因检测花粉飘逸。

Herbicide resistance bar gene was used as selectable marker to test the pollen dispersal .

稀土元素标记基因探针半定量检测HCVRNA

Semi-quantitative Measurement of HCV RNA Using A Europium Labelled Gene Probe

铕标记基因探针半定量检测丙型肝炎病毒RNA

Semi-quantitative measurement of HCV RNA by Europium-labeled gene probe

经Southern杂交分析，证明标记基因lacZY存在于转化菌株的染色体上；

Southern blotting analysis showed that lac ZY gene was inserted into the genome DNA of target strains .

利用标记基因检测转Bt基因棉种子纯度的研究

Technique for Testing Seeds Purity of Bt Transgenic Cotton by Using Marker Gene

陆地棉多标记基因系SSR分析

Analysis of SSR on Tow Multiple-marker Lines in Upland Cotton

当以cat基因作为选择性标记基因时，氯霉素可以作为紫菜原生质体基因转化的选择压力。

Chloramphenicol could be used as selection pressure for positive transformant when cat gene was used as selectable marker gene .

侧连标记基因型下QTL基因型的条件概率

Conditional Probabilities of QTL Genotypes Under Flanking Marker Genotypes

基于BSA分析法的分子标记基因定位技术在农作物中的应用

The Application of the Molecular Gene Localization Technique Based on BSA in Crop

无HindⅢ位点新霉素磷酸转移酶标记基因的建立

Construction of a neomycin phosphotransferase gene without hind ⅲ site

把带有甜蛋白基因和胭脂碱合成酶标记基因的Ti质粒导入马铃薯，获得大量转化植株。

A number of transgenic plants were obtained using the plasmid containing a sweet protein NPT ⅱ and nopaline synthase genes .

Bar基因是广泛应用于基因工程育种中的除草剂抗性基因，也是遗传转化中的标记基因。

Bar gene is widely used in plant genetic engineering breeding as a herbicide resistant gene , and in genetic transformation as a marker gene .

利用DNA免疫法制备植物选择标记基因hpt表达蛋白抗体的研究

Study on the preparing of polyclonal antibodies against plant-selected maker gene hpt expression protein by DNA immunization

目的在肿瘤基因治疗研究中建立一个用绿色荧光蛋白（greenfluorescentprotein，GFP）为标记基因的合适条件。

Objective To search the appropriate experimental conditions for using green fluorescent protein ( GFP ) as a reporter gene in tumor gene therapy .

20个SSR标记基因型与亲本产量及其构成性状配合力显著相关。

Twenty SSR marker genotypes were significantly associated with combining ability of 5 yield and yield components traits .

用hMT-Ia基因作选择标记基因在BPV载体中表达HBsAg的研究

Using bMT-Ia Gene as Selective Marker to Express HBsAg in a BPV Vector

根据RAPD特异分离谱带确定了各品种的标记基因型，以此可实现对美国山核桃无性系品种DNA水平上的鉴别；

The marker gene of each variety was identified based on RAPD strip , therefore , the American hickory clones can be identified on the DNA level ;

菌株质植中GUS基因为标记基因，NPTⅡ基因为选择基因。

GUS gene is as marker gene and NPT ⅱ gene is as selecting gene .

2天后，用荧光显微镜观察标记基因EGFP的表达。

Expression of the marker gene EGFP was observed by fluorescent microscopy 2 days later .

可视监测Cre酶在大肠杆菌中的重组活性及转基因植物中标记基因的消除

Visible Assay for Cre Recombinase Activity in E.coli and Elimination of Marker Genes from Transgenic Plants

从这107对SSR引物中挑选出扩增效果较好的48对，对容量为190个单株的F2群体进行标记基因型分析。

Choose from these primers on SSR primers from 48 to capacity for 190 plants of F2 group type analysis marker genes .

用CFP标记基因法研究菜豆根瘤菌的竞争性和有效性

The Competitiveness and Effectiveness of Phaseolus Rhizobia Studied by Using CFP Marker Gene Technique

该指数可以度量标记基因和性状位点间连锁不平衡（LD）程度。

The index can quantify the level of linkage disequilibrium ( LD ) between the marker and the disease susceptibility locus ( DSL ) of genes .

转sck基因水稻中标记基因表达蛋白HPT食用安全性的初步研究

Study on the food safety of the marker gene expressed protein HPT

植物选择标记基因hpt在大肠杆菌中的融合表达、纯化及活性测定

Fusion Expression , Purification and Bioactivity Assay of Plant Selectable Marker Gene hpt in E. coli

首先将筛选获取的6个连锁群标记基因的SSR分子标记进行重复验证3次，均可得到稳定的结果。

At first , 3 times repeats test of these SSR molecular markers can get the same stable result , which demonstrate the small experimental error .

DNA重组激活基因（Recombinationactivatinggenes，RAG）是脊椎动物特异性免疫反应的关键基因，也是脊椎动物进化分析的标记基因之一。

Recombination activating genes ( Rag ) are key genes in specific immunity system of vertebrate and also used as one of the molecular marker for analysis of vertebrate evolution .

不同加工条件下转基因大米潮霉素标记基因（hpt）稳定性研究

Stability of hpt marker gene in transgenic rice in different food matrices and under varying food-processing conditions