斑蝥素

RP-HPLC法测定斑蝥素脂肪乳剂中斑蝥素的含量

Quantitative Determination of Cantharidin Lipid Emulsion by HPLC

斑蝥素诱导人肺癌A549细胞凋亡及其分子机制的研究

Apoptosis induced by cantharidin in human pulmonary carcinoma cells A549 and its molecular mechanisms

采用体外细胞毒性和体内全身急性毒性试验方法，评价去甲斑蝥素微乳的抗肿瘤活性及其生物安全性。结果微乳平均粒径为（44±9）nm。

Results The mean particle diameter of microemulsion was ( 44 ± 9 ) nm .

顶空GC-MS法测定去甲斑蝥素中的有机溶剂残留

Determination of Residual Organic Solvents in Norcantharidin by Headspace GC-MS

去甲斑蝥素通过半胱氨酸天冬氨酸酶诱导HeLa细胞凋亡

Norcantharidin induces HeLa cells apoptosis through caspases pathway

去甲斑蝥素杀伤HeLa细胞机制研究

Study on Mechanism of Norcantharidin Killing HeLa

结论：MAPK信号传导途径参与了斑蝥素对人肺癌A549细胞的增殖抑制作用。

CONCLUSION : MAPK signal transduction pathway participates the proliferation inhibition of cantharidin on A549 cell .

HPLC-MS法测定大鼠血浆中去甲斑蝥素的浓度

Determination of plasma concentration of norcantharidin in SD rats by HPLC-MS

斑蝥素通过MAPK信号传导途径对A549细胞增殖抑制作用的研究

Study of proliferation inhibition effect of cantharidin on human lung carcinoma A549 cell through MAPK signal transduction pathway

目的：建立大鼠体内去甲基斑蝥素的HPLC-MS分析方法。

Objective : To establish an HPLC-MS method for the determination of norcantharidin in rat plasma .

应用TUNEL标记、DNA凝胶电泳以及光镜技术探讨去甲斑蝥素诱导人T淋巴细胞白血病细胞株（6TCEM）凋亡规律。

To explore the apoptosis of human acute T lymphocyte leukemia cell line 6T CEM induced by Norcantharidin with TUNEL labeling , DNA gelelectrophoresis and microscopy .

采用复乳法和相分离法两种方法制备去甲斑蝥素的聚乳酸-聚乙二醇嵌段共聚物（PLA-PEG）纳米微球。

Norcantharidin ( NCTD ) loaded PLA-PEG nanoparticles are prepared by double emulsion method and nanoprecipitation method respectively .

方法用小鼠半数致死量（LD50）及生化指标的变化来表征斑蝥素的毒性，用小鼠急性死亡率法测定斑螫素药物动力学参数。

METHODS The toxicity of cantharidin was expressed at lethal dose 50 and biochemical indexes , apparent pharmacokinetic parameters of cantharidin were determined by using acute mortality of mice .

目的观察去甲基斑蝥素（NCTD）体内外对人卵巢癌细胞株3AO和AO的抑制作用。

Objective To evaluate the inhibitory effects of Norcantharidine ( NCTD ) on human ovarian cancer3AO and AO cell lines in vitro and in vivo .

目的：探讨去甲斑蝥素诱导人T淋巴细胞白血病细胞株6T-CEM的凋亡作用。

To explore the role of Norcantharidin in inducing apoptosis of human acute T lymphocyte leukemia cell line 6T CEM .

目的通过斑蝥素对G1/S期肾小管上皮细胞（RTECs）低氧损伤后细胞ATP代谢影响的研究，探讨斑蝥素稳定肌动蛋白微丝骨架系统的机制。

Objectives To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G1 / S-phase renal tubule epithelial cells ( RTECs ) .

背景与目的：去甲斑蝥素（norcantharidin，NCTD）是斑蝥素（cantharidin）的衍生物，对多种肿瘤细胞的生长均有抑制作用，但其药效较同类化疗药物小。

BACKGROUND & OBJECTIVE : Norcantharidin ( NCTD ), the demethylated form of cantharidin , can inhibit the proliferation of many kinds of cancer cells , but its effect is milder than those of other drugs .

目的探讨去甲斑蝥素-海藻酸/聚酸酐微球（norcantharidin-alginicacid/polyacidanhydridemicro-spheres，N-MS）介入治疗大鼠肝癌的作用及其机制。

Objective : To investigate the effects of interventional therapy with norcantharidin-alginic acid / poly acid anhydride microspheres ( N-MS ) infusion via hepatic artery on hepatoma in rats .

源于中药的天然产物在治疗癌症中有很大潜力，例如青蒿琥酯，高三尖杉酯碱，As2O3，斑蝥素。对照临床研究发现，高三尖杉酯碱和As2O3对治疗白血病有显著活性。

TCM natural products have the potential for use in cancer therapy , for example ; artesunate , homoharringtonine , arsenic trioxide and cantharidin , the controlled clinical studies have shown that homoharringtonine and arsenic trioxide can exert profound activity against leukaemia .

结论：一定剂量范围内的去甲斑蝥素可在直接杀伤作用较小的情况下明显诱导6T-CEM细胞凋亡，不会因细胞坏死而引起炎症反应，这对于去甲斑蝥素应用于临床将有十分重要意义。

Conclusion : Dosage of Norcantharidin was still within the range of minor directly killing efficacy , which had markedly induced apoptosis of 6T CEM cells , without inflammation caused by cell necrosis , thus great importance is borne by clinical application of Norcantharidin .

去甲斑蝥素-泊洛沙姆407缓释制剂局部注射治疗肿瘤的实验研究

Experimental Study on Intratumoral Administration with Sustained-Release Preparation of Norcantharidin-Poloxamer 407

去甲斑蝥素诱导人肾癌786-0细胞凋亡的体外研究

Studies on norcantharidin-induced apoptosis on renal carcinoma 786-0 cell in vitro

斑蝥素致深度化学烧伤1例

Deep chemical burn due to cantharidin : report of one case

去甲斑蝥素诱导人T淋巴细胞白血病细胞株凋亡

Norcantharidin-induced apoptosis of cell line 6T-CEM from acute T lymphocyte leukemia

斑蝥素对菜蛾的毒杀作用研究初报

The insecticidal function of cantharidin to the larvae of diamondback moth

天然源农药0.1%斑蝥素水溶剂的应用技术

Study on Applied Techniques of Natural Watery Pesticide 0.1 % Cantharidin

桔梗细胞悬浮培养体系对斑蝥素的生物转化研究

Biotransformation of cantharidin by cell suspension cultures of Platycodon grandiflorus

去甲斑蝥素用于单克隆抗体为载体的肿瘤导向治疗实验研究

Use of Monoclonal Antibody Norcantharidin Conjugate in Experimental Targeting Therapy of Tumor

去甲斑蝥素脂质体的制备工艺优化及其药剂学性质研究

Preparation Technique Optimization of Norcantharidin Liposome and Its Pharmaceutical Properties

动物源农药斑蝥素对部分非靶标生物的安全性评价

Safety evaluation of animal-origin pesticide cantharidin against some non-target organisms