平板培养基
- 网络plating medium;culture plate
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本文对从野外获得的5株菌株进行了生物学特性比较,从设计组合中筛选出最优良菌株及最佳平板培养基。
In order to screen out the best strain and plating medium , explore the biological character of the five strains obtained from the field .
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通过子实体组织分离的菌种,接种于PDA和MEA平板培养基上,在25℃恒温下培养1~6周。
By isolating the fructification tissue , the cultures were inoculated in petri dishes on PDA medium and MEA medium , they were incubated at 25 ℃ for 1 ~ 6 weeks .
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对9株发酵乳球菌菌株进行MRS固体平板培养基划线分离和形态学比较观察,并采用生化试验和RAPD对其进行鉴定。
In this paper , the isolation and identification of 9 lactic acid coccus strains from fermented milk were studied . The lactic acid bacteria were isolated by MRS plate streaking cultivation and morphological observation , and were identified by biochemical essay and RAPD technique .
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结果表明:As-1在含硒平板培养基中,当硒含量达5μg/ml时菌丝生长几乎不受抑制;
The results showed that in the solid media , the fungus-AS-1 growth was hardly inhibited when the concentration of sodiun selenite was 5 μ g / ml.
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研究结果表明,在固体平板培养基上,菌株M1的生长速度较快;在液体发酵过程中,菌株M1发酵液的滤纸纤维素酶、漆酶和多酚氧化酶的活性均高于其它4个菌株。
The results indicated that growth rate of rhizomorphs of strain M_1washigher than others in the solid medium , and the activation of cellulase , laccase , polyphenol oxidase from strain M_1were higher than that of the other strains in liquid culture .
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将培养好的菌液平铺于含有抗生素和X-gal、IPTG的平板培养基中,通过抗药性筛选及蓝白斑试验挑选所需的大肠杆菌,继续于液体培养基中过夜培养。
The bacterio-liquid was spreaded on the plate culture medium contained the Aminobenzylpenicillin , the X-gal and IPTG . ⑦ Then , we picked out the needed germs by drug-resistance screening and the blue-white spots test .
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方法用罗氏固体平板培养基,分别采集医院内中央空调控温区、分体空调控温区和无空调区的空气标本,经36.5℃培养28d。
Methods The air-samples in the areas with central air-condition , and independent air-condition and no air-condition were collected and incubated in the Lowenstein-Jensen culture medium separately . The air-samples have been cultivated in 36.5 ℃ for 28 days .
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文中描述了它们在察氏平板培养基上25℃恒温培养3星期的菌落特征。
In this paper , their colony characteristics on Czapek medium under 25 C for 3 weeks were described .
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在平板培养基、液体培养基内分别处理这些微生物,蘑菇菌丝体生长速度加快,菌丝量增加。
Adding these microbes into PDA solid and liquid media , the growth rate and biomass of mycelium were increased .
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并通过正交试验确定菌株的最佳培养基为干酪素平板培养基,水解酪蛋白试验可对菌株的产酶情况进行定性研究,酶活较高的菌株都能产生较大的水解圈。
Through orthogonal test , it was shown that casein plate medium was the optimum one for the strains . Research on the enzymes-producing strains was conducted through test of casein hydrolysis .
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方法采用专用巧克力和血平板培养基对采集的400名3~5岁儿童鼻咽分泌物培养并鉴定,对鉴定明确的肺炎链球菌和流感嗜血菌进行不同抗生素检测。
Methods Four hundred samples of rhinopharynx secretion from children 3 ~ 5 years old were cultured with special chocolate and blood medium plate . Sp and Hi isolated were tested for antibiotic susceptibility .
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曲霉糖化酶高产菌株平板分离培养基的筛选
Selection of Flat Separation Culture Medium for High-yield Strains of Saccharifying Enzymes from Aspergillus
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将液体培养物稀释到同一梯度,定量接种于固体培养基上(绵羊血琼脂平板和特殊培养基),分别进行需氧和厌氧培养24~48h。
Things of the above cultured , after diluted to the same gradient and inoculated on solid culture mediums quantitatively , were cultured for 24 ~ 48 h under the condition with oxygen and without oxygen .
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方法:采用鸡蛋斜面培养基,亚碲酸钾血琼脂平板和肉汤培养基观察其生长特性、测定多种生化反应性、毒力和药物敏感性。
Methods : To observe the cultural characteristics with the medium of inclined plane of egg , agar plate with blood and potassium tellurite , and broth .
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采用MRS培养基的双层平板法和MRS培养基的试管法可对嗜热链球菌进行选择性计数;
The method of double layer MRS medium and MRS medium in test tube can enumerate Streptococcus thermophilus selectively ;
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通过羧甲基纤维素琼脂平板、滤纸条培养基从自然环境中筛选到4株高效纤维素降解菌,其中木霉2株,青霉2株,分别记为T-1、T-2和P-1、P-2。
Species of fungi including 2 species of Trichoderma T-1 , T-2 and 2 species of Penicillium P-1 , P-2 were selected from nature environment via carboxymethyl cellulose agar plate and filter paper medium .