基因敲除

本文综述了基因敲除动物在各医学生物领域的研究与应用、浅谈其与RNA干扰技术的比较及其发展前景。

The study and application of gene knock-out animal were reviewed in this article . Comparison between gene knock-out technique and RNA interference technique was also overviewed .

细胞周期蛋白D1基因敲除对小鼠脑缺血边缘区胶质细胞增殖及神经元凋亡的影响

Effects of cyclin D1 gene knock-out on astrocyte proliferation and neuronal apoptosis in boundary zone following the focal cerebral ischemia

Red重组系统在痢疾杆菌基因敲除中的应用研究

Study on Gene Knockout Using Red System in Shigella flexneri

利用Red同源重组系统进行牛β酪蛋白基因敲除

Knock out of bovine beta casein gene by homologous recombination

多巴胺D2受体基因敲除对鼠肾上腺素和内皮素B受体依赖性高血压的影响

Disruption of the D_2 dopamine receptor produces adrenergic and endothelin B receptor dependent hypertension

载脂蛋白E基因敲除小鼠冠状动脉内粥样硬化病灶的研究

Study on the atherosclerotic lesions in coronary artery of apoE gene knockout mice

不同周龄载脂蛋白E基因敲除小鼠肾脏损伤的进展情况

Renal Injury in Apolipoprotein E-Deficient Mice of different ages

不同应激对载脂蛋白E基因敲除小鼠动脉粥样硬化及斑块炎症反应的影响与意义的研究

Effect of Different Type of Stress on Atherosclerosis and Inflammation in Apolipoprotein E Gene Deficient Mice

炎症诱导肝细胞内脂质堆积及载脂蛋白E基因敲除小鼠肪肝形成

Inflammatory stress exacerbates lipid accumulation in hepatic cells and fatty liver of apolipoprotein E knockout mice

免疫球蛋白Fc区介导抑制脂蛋白E基因敲除鼠动脉粥样硬化形成

The Fc region of immunoglobulin suppresses atherosclerosis in apolipoprotein E knockout mice

载脂蛋白E及其基因敲除小鼠的研究进展

ApoE and ApoE Knockout Mice

结论套环能加速载脂蛋白E基因敲除小鼠颈总动脉斑块形成。

Conclusions Perivascular common carotid collar placement can accelerate atherosclerotic plaque formation in apolipoprotein E-knock out mice .

化合物48/80对载脂蛋白E基因敲除小鼠主动脉斑块的影响

Effect of Compound 48 / 80 on Thoracic Aortic Plaque in Apolipoprotein E - knock out Mice

CD4和CD8基因敲除鼠行穿透性角膜移植术后免疫排斥特征的研究

The corneal allograft rejection features in CD4 and CD8 knock-out mice

人DNA聚合酶Polι体细胞基因敲除载体的构建

Construction of Human Pol ι Somatic Gene Targeting Vector

RNA干扰与基因敲除

RNA interference and gene knocking out

crt基因敲除可损伤心脏发育而导致胚胎死亡。

Knockout of crt gene is embryonically lethal due to impaired cardiac development .

L1CAM基因敲除小鼠的脑局部血流量、ATP含量和蛋白合成率分析

Analysis of focal cerebral blood flow , ATP content and protein sythesis of L1 gene knockout mice

同时，课程也将对RNA干扰和基因敲除等重要的实验技术的原理及应用作专题介绍。

In addition , several useful laboratory techniques , e.g.small RNA interference and gene knockout are comprehensively introduced during the lecture .

基因敲除技术是建立在同源重组（HomologousRecombination，HR）基础之上对基因组进行遗传修饰的一种较理想的方法。

Gene targeting is a useful protocol to modify the cell genome based on the homologous recombination .

ApoE基因敲除小鼠认知功能障碍的Morris水迷宫观察

Cognitive impairment displayed by ApoE knockout mice in the Morris water maze

植物乳杆菌对IL-10基因敲除结肠炎小鼠黏附分子的影响

Lactobacillus plantarum attenuates up-regulation of adhesion molecules expression in IL-10 gene knockout mice with colitis

在SR－AI／I基因敲除小鼠，OX－LDL在血浆的清除速率和正常小鼠相同。

Clearance rates of oxidized LDL were equally high in SR-AI / II gene knockout mice .

ApoE基因敲除鼠12周游泳运动前后纤溶激活功能的改变

Plasminogen Activation Changes in ApoE Gene Deficiency Mice after 12-week Swimming

βB2晶体蛋白基因敲除小鼠模型的建立

Establishment of a β B2 crystallin gene knockout mice model

基于此种现状，本课题拟用化合物48/80探讨肥大细胞脱颗粒与载脂蛋白E基因敲除小鼠主动脉斑块的关系。

Based on the status , compound 48 / 80 and apolipoprotein E-knockout mice were used to investigate the effect of mast cell degranulation on plaque .

这些结果表明Pten基因敲除小鼠骨吸收增加。

These results indicated that disruption of Pten in osteoblasts leads to increased bone resorption .

CD8基因敲除小鼠在（28±3）d时发生免疫排斥反应；

The corneal grafts in CD8 knock out mice were rejected at ( 28 ± 3 ) days .

在这些突变中SOD酶的活性基本正常或升高，SOD基因敲除的小鼠则没有发病。

For many of these mutations , SOD1 enzyme activity is actually normal or elevated , and SOD1 knockout mice have no disease phenotype .

雌激素对D-半乳糖去卵巢小鼠及其apoE基因敲除鼠认知能力的影响

Influence of estrogen on the cognition in D-galactose-induced ovariectomized mice and their apoE-knock-out mice