反转录病毒

猪内源性反转录病毒囊膜蛋白基因的克隆及其蛋白二级结构和B细胞表位预测

Clone and Prediction of the Secondary Structure and B Cell Epitopes for the Env Protein of Porcine Endogenous Retrovirus

J亚群禽白血病病毒（ALV-J）是一种主要感染肉用型鸡的反转录病毒。

Avian leukosis virus subgroup J ( ALV-J ) is a retrovirus which infects meat-type chickens .

反转录病毒载体G1NaCⅨ的构建及其在离体细胞中的表达

Construction of retrovirus vector G_1NaC ⅸ and its expression in cells in vitro

肿瘤的基因治疗前期研究：反转录病毒介导的肿瘤坏死因子α基因抗C6胶质瘤效应

Early investigation of gene therapy for cancer : effect of tumor necrosis factor alpha induced by retrovirus again C_6-glioma

用RACE技术快速扩增五指山猪来源的猪内源性反转录病毒3'LTR

Sequence determination and analysis of 3'LTR of porcine endogenous retrovirus using rapid amplification of cDNA ends

反转录病毒载体介导可溶性Fas的体外表达

Retroviral Vector - Mediated In Vitro Expression of Human Soluble Fas

反转录病毒介导的野生型p53基因在人喉癌等肿瘤细胞中的表达及对肿瘤生长的抑制作用

Expression of Retroviral Mediated Wild Type p53 Gene in Laryngocarcinoma Cell Lines and Its Inhibition Effects to Cancer Growth

一DXFD（52）相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建

Partial Sequence Analysis and Construction of Recombinant Retroviral Vec-tor of a DXFD_ ( 52 ) - Related Human Hepatocyte CDNA

现在普遍认为DNA的甲基化的功能是参与许多印记基因、失活的X染色体上的基因、转座子和内源的反转录病毒的沉默。

It is believed that DNA methylation has important functions in gene imprinting , for transposons and in vivo retrovirus silencing as well as the stability of the genome ;

J亚群禽白血病病毒属于反转录病毒禽白血病／肉瘤病毒群。该病毒是由英国Dr。

Avian leucosis virus subgroup J ( ALV-J ) belonging to leucosis / sarcoma group of retrovirus was discovered in 1991 by Dr.

反转录病毒载体介导的EGFP基因在SK-N-SH神经母细胞瘤细胞中的表达

Retroviral vector mediated expression of EGFP gene in SK-N-SH neuroblastoma cells

方法：采用反转录病毒介导的基因转移和ACV体内治疗方法进行研究。

Methods : Retroviral vector / ACV system were used .

HSV-tk基因反转录病毒载体的构建及体外抗脑肿瘤细胞作用研究

Study on Constructing Retroviral Vector Carrying HSV-tk Gene and Its Antitumor Effect in Vitro

电镜观察可见Gag蛋白在CEF细胞中形成的反转录病毒样粒子。

The virus like particles formed by Gag protein were observed under electron microscope .

本文还就在反转录病毒介导的基因转移过程中tk基因突变可能导致C6/NeoR+TK-细胞的出现进行了讨论。

C6 / NeoR + TK - might caused by the mutation of tk gene in the retrovirus mediated gene transfer .

猪内源性反转录病毒gag基因的克隆及其在大肠杆菌中的表达

Cloning of gag Gene and Its Expression in E.coli of Porcine Endogenous Retrovirus Derived from Chinese Experimental Miniature Pigs

含HIV-1Tat基因重组反转录病毒表达载体构建与表达Tat蛋白功能检测

Construction of a recombinant retroviral vector containing HIV-1 Tat gene and functional detection of the expressed Tat in target cells

马传染性贫血病毒（EquineinfectiousanemiaVirus，EIAV）属反转录病毒科慢病毒属，是马传染性贫血（EIA，简称马传贫）的病原体。

Equine infectious anemia virus ( EIAV ) is a lentivirus within the family Retroviridae and the causative agent of equine infectious anemia ( ElA ) .

利用重组反转录病毒表达马立克氏病病毒Meq基因

Expression of Marek ′ s Disease Virus Meq Gene by the Recombinant Retrovirus

目的构建含HIV1Tat基因重组反转录病毒表达载体及评价表达Tat蛋白的功能。

Objective To construct a recombinant retroviral vector containing HIV-1 Tat gene and evaluate the function of the expressed Tat in target cells .

牛免疫缺陷病毒（Bovineimmunodeficiencyvirus，BIV）在分类上属于反转录病毒科的慢病毒属，目前尚未见BIV感染人的报道。

Bovine immunodeficiency virus ( BIV ) is a kind of Lentivirus belonging to Retrovirus , and up to now , there is no report about its infection to human .

使用的病毒可以是腺病毒、AAV、反转录病毒、疱疹病毒。

The viruses that are commonly used for this purpose are adenovirus , adeno-associated virus ( AAV ), retrovirus , lentivirus and herpes virus .

在果蝇中，杏黄色眼基因w~a和它的野生型白眼等位基因w的区别在于前者的转录单位中插入了反转录病毒样转座因子copia。

The white-apricot ( w α) allele differs from the wild type white gene by the presence of the retrovirus-like transposable element copia within the transcription unit .

反转录病毒前病毒基因组长末端重复序列（LTR）对病毒复制有重要的调控作用，影响到病毒的复制动力学、细胞嗜性、致病力等方面。

The EIAV proviral genomic long-terminal repeat ( LTR ) is a eukaryotic promotor , which plays an important regulatory role on transcription and replication of the virus .

目的克隆分析中国特有小型猪来源的猪内源性反转录病毒（PERV）囊膜基因（env），比较不同来源毒株的异同及系统进化关系。

To analyze the interrelation and variation between the envelope gene of porcine endogenous retrovirus from miniature swine of China and other PERV published strains .

目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。

Objective To construct the retroviral ( RV ) vector with report gene enhanced green fluorescent protein ( EGFP ) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells .

在过去的20年里，出现了一些新的转基因方法，包括精子介导、反转录病毒介导、携带外源基因体细胞的核移植、ES细胞基因打靶技术等。

In the past 20 years , some new approaches have been used . These include sperm mediated DNA transfer , retroviral mediated DNA transfer into oocytes , somatic cell carrying exogenous genes nuclear transfer , the use of embryonic stem ( ES ) cell .

目的研究反转录病毒介导的绿色荧光蛋白（EGFP）基因和酪氨酸羟化酶（TH）基因在原代培养的骨髓基质细胞（bMSCs）中的表达情况。

Objective To investigate the expression of enhanced green fluorescent protein ( EGFP ) and tyrosine hydroxylase ( TH ) genes in bone marrow stromal cells ( bMSCs ) . Methods bMSCs were isolated by a density gradient ( lymphocytes separation ) from limbs .

水稻东格鲁病是由水稻东格鲁杆状病毒（RTBV）和水稻东格鲁球状病毒（RTSV）复合感染引起的.RTBV是一种杆状外型的植物副反转录病毒；

Rice tungro is caused by Rice tungro bacilliform virus ( RTBV ) and Rice tungro spherical virus ( RTSV ) in a composite manner .

目的：为了保护骨髓免受或少受化疗药物的损伤，用转染人类突变二氢叶酸还原酶（DHFR）基因反转录病毒载体，研究其耐药特性及在小鼠造血细胞的表达和对造血细胞的保护作用。

Objective : To protect the bone marrow cells from chemotherapeutic injuries , using retroviral vector of mutant human dihydrofolate reductase gene , the paper investigated its drug resistant characteristics and for murine hematopoietic cells transduction and protection .