双脱氧核苷酸

DNA序列测定&M13双脱氧核苷酸序列分析系统

DNA SEQUENCE DETERMINATION The M13 Dideoxynucleotide Sequencing System

方法应用荧光标记双脱氧核苷酸进行单碱基延伸反应，同步检测人类DRB基因10个单核苷酸多态性位点。

Methods Using fluorescent dye labeled ddNTP , the single nucleotide extension ( SNE ) reaction was conducted to analyze 10 SNP loci in human DRB gene .

方法采用错配引物介导的聚合酶链反应／限制性酶切分析法和双脱氧核苷酸指纹印迹检测法，对天津地区22例G6PD缺陷患者进行3种中国南方人群常见的G6PD基因突变的分析。

Methods DNA samples from 22 unrelated patients ( 21 male and 1 female ) with G6PD deficiency from Tianjin area in North China were studied for the three common mutations by using both mismatched primers mediated polymerase chain reaction / restriction enzyme analysis and dideoxy fingerprinting methods .

扩增产物经纯化后通过一个以链终止法为基础的循环测序程序直接进行序列测定，其中所用四种双脱氧核苷酸分别带不同的荧光标记，测序引物选自同一组通用引物。

Products of amplification were directly sequenced by chain termination-based cycle sequencing protocol after being purified , in which four ddNTPs were separately labeled with four different fluoresceins , and primers used for PCR amplification were reemployed as sequencing primers .