双孢蘑菇

双孢蘑菇储藏中的褐变及相关酶活性研究

Enzymes Associated With Browning of Agaricus bisporus Fruit Bodies During Storage

双孢蘑菇风味的强化与胶囊化研究

The Enhancement and Encapsulation of Agaricus Bisporus Flavor

中国主栽双孢蘑菇菌株的DNA多态性

DNA Polymorphism of Major Cultivation Strains of Agaricus bisporus in China

双孢蘑菇栽培菌株遗传多样性的DNA指纹分析

DNA fingerprinting of genetic diversity of Agaricus bisporus cultivation strains

双孢蘑菇原生质体同核体及杂交异核体的RAPD分析

RAPD Analysis of Protoplasted Homokaryons and Hybrid Heterokaryons of Agaricus bisporus

双孢蘑菇RAPD标记的遗传多样性分析

Detection of Genetic Diversity of Agaricus bisporus Using RAPD Markers

应用RAPD技术分析两个双孢蘑菇遗传家系

RAPD analysis of two genetic families of Agaricus bisporus

可溶性蛋白含量测定表明：供试双孢蘑菇菌株之间可溶性蛋白质含量相差较大，差异最大的可达14.251μg／μL；

The content of soluble protein of the tested strains showed great difference among them .

检测双孢蘑菇培养料中微量1-氨基环丙烷-1-羧酸（ACC）的离子色谱法

Ion Chromatography for Determination of Trace Amount 1-Aminocyclopropane-1-Carboxylic Acid ( ACC ) in Mushroom

提高双孢蘑菇（Agaricusbisporus）生产效益的新技术

A new technique in increasing the production of Agaricus bisporus

双孢蘑菇品种间RAPD遗传多样性与亲缘关系的研究双孢蘑菇、野蘑菇和姬松茸的过氧化物酶和酯酶的同工酶分析

Study on RAPD Genetic Diversity and Relationship among the Strains of Agaricus Bisporus ; Analysis of POD and EST Isozyme of Three Agaricus Species

本研究是在形态学观察的基础上，利用了重复序列间区DNA标记和随机扩增多态性DNA标记等技术，研究了侧耳属的22个菌株和一株双孢蘑菇菌株的系统发育关系。

Based on morphological taxonomy , we applied a new maker of ISSR and conventional one of RAPD to study the molecular taxonomy of 22 isolates of the genus Pleurotus and one of Agaricus bisporus .

本研究以双孢蘑菇原生质体同核体及杂交异核体为材料，用5对随机双引物进行RAPD分析。

Random Amplified Polymorphic DNA ( RAPD ) analysis was conducted with 5 random paired primers in protoplasted homokaryons and hybrid heterokaryons of Agaricus bisporus in this study .

因此，RAPD分析是一种快速有效的手段，能够在双孢蘑菇的遗传研究和菌种改良中发挥重要作用。

It was suggested that RAPD analysis was a useful technique , and that it could play an important role in genetic research and strain improvement of Agaricus bisporus .

本研究用双孢蘑菇2796为试样，采用RT-PCR、RACE法克隆了来自于双孢蘑菇子实体褐化相关的PPO基因，并使其在大肠杆菌中做表达。

This study used 2796 as Agaricus bisporus samples using RT-PCR , RACE cloning from the fruiting bodies of Agaricus bisporus PPO browning-related genes , and its expression in E.coli .

双孢蘑菇多糖对鸡肝在37℃恒温腐败培养12h以内无明显抑制；

Agaricus bisporus ( Lange ) Sing polysaccharide in the chicken liver 37 ℃ thermostatic without obvious curb corruption within a 12h .

得到的两种重组表达蛋白的分子量分别为71kDa和69kDa，均能被羊抗双孢蘑菇PPO抗体所识别。

The molecular weight of the reconbinant proteins were 71 kDa and 69 kDa , respectively . Both of them could be probed by the sheep anti-Agaricus bisporus PPO antibody .

而贮藏过程中温度波动将导致双孢蘑菇采后开伞率、呼吸强度提高，PPO活性和褐变度增加，对其采后生理和品质造成严重影响。

Temperature fluctuation during storage may result in very bad consequence , because it will increase the rate of cap opening , the activity of PPO and the degree of browning .

以提高双孢蘑菇的保鲜质量为目的，分析了O3气体处理时间对保鲜蘑菇的细胞膜透性、PPO和CAT活性的影响。

To improve the fresh-keeping quality of Agaricus bisporus , the effect of the treating time with ozone on plasma membrane permeability , PPO activity and CAT activity of fresh-keeping Agaricus bisporus were analyzed .

本研究以收集自世界各地的206个双孢蘑菇栽培菌株为材料，进行栽培出菇筛选试验和总DNA大规模的SRAP、ISSR和RAPD分析。

In this paper , 206 cultivation strains of Agaricus bisporus collected from all over the world were studied by screening test of cultivation and using the techniques of SRAP , ISSR and RAPD fingerprinting .

结果表明：适合MA气调贮藏的双孢蘑菇直径大小为30～40mm；

The results showed that the optimum range of Agaricus bisporus diameter was 30 ~ 40 mm for MA storage ;

结果表明：在相同条件下，O3气体处理10min的双孢蘑菇保鲜效果相对较好，表现为细胞膜透率和PPO活性受到有效抑制，CAT活性保持在较高水平。

The result showed that Ozone treatment for 10 minutes produced the best result , showing that the plasma membrane permeability and PPO activity were inhibited , CAT activity kept higher level .

用72个随机引物，对三种类型双孢蘑菇菌株2796（杂交型）、8211（优质型）、01（高产型）等进行RAPD扩增，发现26个引物均产生三种不同的带型。

Three types of A . bisporus strains including 27 % ( hybrid type ), 8211 ( excellent - quality type ) and 01 ( high - yield type ) were studied hy RAPD amplification reaction with 72 random primers .

双孢蘑菇分解基质能力退化菌株的DD-RTPCR分析

DD-RTPCR analysis of the substrate degradation ability degenerating strains of Agaricus bisporus

将双孢蘑菇176、2796、8211菌株分别培养在用普通水配制的马铃薯培养基（PDA）和用磁化水配制的马铃薯培养基（MPDA）上。

Agaricus bisporus strain 176 , 2796 , 8211 were inoculated in the potato dextrose agar made up with water ( PDA ) and magnetized water ( MPDA ) .

另外应用荧光差异显示技术（FluroDD-PCR）获得几条双孢蘑菇菌株02菌丝体的高温诱导特异表达基因片段，将它们克隆并测序。

Another method , Fluro DD-PCR was also used and several thermotolerance related differential DNA fragments of strain 02 were cloned and sequenced .

双孢蘑菇气调包装呼吸速率模型与品质变化试验研究

Studies on Respiration Rate and Quality of Atmosphere Packaged Agaricus Bisporus

双孢蘑菇褐腐病综合防治技术研究

A Study on the Integrate Control of Agaricus bisporus Wet Bubble

反义酪氨酸酶基因抑制双孢蘑菇褐变的研究

Studies on inhibition of Agaricus bisporus browning by antisense tyrosinase gene

双孢蘑菇冻干的升华与解析工艺研究

Research on the Distilling and Resolving Technology of lyophilizing Agaricus bisporus