单细胞凝胶电泳技术

应用碱性单细胞凝胶电泳技术（SCGE）检测镉致鸡脾淋巴细胞DNA的损伤效应

Effects of Cadmium on DNA Damage in Chicken Splenic Lymphocytes with Alkaline SCGE

分别应用中性单细胞凝胶电泳、碱性单细胞凝胶电泳技术检测了不同剂量γ射线外照射对小鼠外周血淋巴细胞DNA的损伤。

DNA single-and double-strand breaks of peripheral blood lymphocytes in mice induced by γ rays irradiation were detected by neutral and alkaline comet assay .

单细胞凝胶电泳技术检测蜘蛛血细胞DNA损伤研究

Study on DNA Damages of Hemocytes in Spider with Single Cell Gel Electrophoresis

改良单细胞凝胶电泳技术在DNA损伤与修复检测中的应用

Application of the technology of single-cell gel electrophoresis improvement to the detection of DNA damage and repair

方法单细胞凝胶电泳技术测定鱼红细胞DNA损伤效应。

Methods Single cell gelatin electrophoresis test ( SCGE Test ) was used to measure DNA trauma of fish red blood cell .

单细胞凝胶电泳技术可作为砷化合物DNA损伤的有效检测手段。

The single-cell gel electrophoresis could be used as an effective method to detect the DNA damage of organoarsenic compound in the environment .

目的单细胞凝胶电泳技术是检测细胞DNA损伤与修复的一种快速、简便、灵敏方法，但影响因素很多，为此对该技术进行了改进，并对操作中易出现的问题进行了总结。

Objective : To improve the technology and summary the problems of single-cell gel electrophoresis in detecting the damage and repair of cell DNA .

单细胞凝胶电泳技术是一种在单细胞水平上检测DNA损伤与修复的技术，它具有快速、灵敏、简便等优点。

The single cell gelelctrophoresis assay is a rapid , simple and sensitive technique for measuring DNA damage and repair at the single cell level .

结论单细胞凝胶电泳技术是一种简便、敏感的检测DNA氧化损伤的方法。

Conclusion Single cell gel electrophoresis technique is a simple and sensitive method to determine oxidation-induced single strand breaks ( SSB ) formation in DNA .

目的用碱性单细胞凝胶电泳技术检测苯作业工人外周血淋巴细胞DNA损伤，研究苯的遗传毒性。

Objective To detect DNA damage in peripheral lymphocytes of workers exposed to benzene by alkaline single cell gel electrophoresis and to study the genetic toxicity of benzene .

单细胞凝胶电泳技术又称为彗星实验，是最近几年发展起来的一种快速、简单、灵敏、可靠的检测细胞核DNA损伤的技术。

The single cell gel electrophoresis technique ( comet assay ) is a simple , sensitive , reliable , and rapid method to detect DNA damage in eukaryotic cells .

方法大鼠灌胃番茄红素4周后，采用单细胞凝胶电泳技术对外周血淋巴细胞和肝细胞DNA损伤进行分析。

Methods Rats were given LP after 4 weeks , the DNA break in peripheral blood lymphocytes and liver cells from the rats were analyzed with single cell gel electrophoresis .

以氯化的商品腐殖酸溶液为实验模型，用单细胞凝胶电泳技术检测了氯化腐殖酸引起的V79细胞DNA损伤与修复作用。

In this paper , chlorination of commercial humic acids was used as an experimental model .

目的探索应用彗星实验（单细胞凝胶电泳技术，SCGE）测定鲤鱼红细胞DNA损伤以评价地面水污染遗传毒性效应的可行性。

Objective To explore the feasibility of assessing the genotoxic effect for surface-water pollution using carp erythrocytes with comet test .

目的探讨整体照射与离体照射导致小鼠淋巴细胞DNA双链断裂的一致性，作为单细胞凝胶电泳技术应用于辐射生物剂量学的前期研究。

Objective To observe the consistency of DNA double-strand break between in vivo and in vitro irradiation , as a prophase study in radiation biodosimetry using single cell gel electrophoresis ( SCGE ) .

为评估农用稀土常乐对人体胚胎的影响，采用微核试验和单细胞凝胶电泳技术检测混合稀土常乐能否通过胎盘屏障造成胎儿细胞DNA损伤。

To assess the potential risk of mixed rare earths Changle for human embryo transplacental micronucleus test and single cell gel electrophoresis ( SCGE ) technique to detect DNA damage of embryo we used .

应用单细胞凝胶电泳技术和液相色谱电化学检测法研究了典型环境污染物乙醛对脱氧核糖核酸（DNA）分子的损伤效应。

The effects of DNA damage induced by the typical environmental pollutant acetaldehyde were studied with single cell gel electrophoresis ( SCGE ) and high performance liquid chromatography with electrochemical detection ( HPLC-EC ) .

目的介绍一种改良的单细胞凝胶电泳技术同时检测血白细胞DNA链断裂和DNA－蛋白交联并将其应用于部分健康人群。

Objective To introduce a modified single cell gel electrophoresis for detecting DNA strand breaks ( DSB ) and DNA-protein crosslinks ( DPC ) in white blood cells simultaneously , and apply it to healthy population .

目的研究F-2毒素引起体外培养细胞（HELA细胞）的DNA损伤。方法应用单细胞凝胶电泳技术（SCGE），测定玉米赤霉烯酮，即F-2毒素。

DNA damage of HELA cells by F-2 toxin was determined by single-cell gel electrophoresis ( SCGE ) .

运用单细胞凝胶电泳技术，研究了太原市大气细颗粒物（PM2.5）对分离的大鼠肺泡巨噬细胞DNA的损伤作用。

Damage from atmospheric fine particles ( PM2.5 ) in isolated DNA of alveolar macrophages of rats in Taiyuan City was studied applying single cell gel electrophoresis technique .

背景与目的：采用单细胞凝胶电泳技术研究壬基酚（nonylphenol，NP）对牡蛎血淋巴细胞DNA的损伤作用。

BACKGROUND & AIM : The effect of nonylphenol ( NP ) on DNA damage in oyster hemolymph cells was measured by single-cell gel electrophoresis assay in vivo .

方法分别以γ射线及紫外线作为标准断裂剂，用单细胞凝胶电泳技术对不同浓度甲醛诱导的小鼠胸腺淋巴细胞DNA交联作用进行体内及体外实验研究。

Methods γ - rays and UVL were used as standard DNA breaking agents , the DNA cross-links in thymocytes of mice induced by formaldehyde were studied in vitro and vivo with single cell gel electrophoresis assay ( SCGE , comet assay ) .

[方法]用单细胞凝胶电泳技术检测阴性对照组、氯化镉组、同时处理组、后处理组及预先处理组大鼠DNA损伤情况。

METHODS The tests were divided into five guoups : control group , Cd groups , co-treatment groups , post-treatment groups and pre-treatment groups . The single cell gel electrophoresis assay was used to inspect the DNA damage of whole hemocytes in rats .

方法用中性单细胞凝胶电泳技术检测1Gyγ射线辐照后不同时间点的小鼠和人淋巴细胞DNA双链断裂，分别拟合DNA修复曲线，并对二者曲线模型进行比较。

Meth - otis The DNA double-strand break in lymphocytes of human and mouse was detected using neutral single cell gel electrophoresis ( SCGE ) after radiation and the curves of DNA repair individually were estimated , which were com - pared later .

应用高效液相色谱电化学检测技术，以及单细胞凝胶电泳技术，研究了11种不同价态金属离子体外诱导核酸（DNA）氧化损伤产生8羟基脱氧鸟苷和DNA链断裂的化学机制。

The oxidative DNA damage markers - 8-hydroxy-2 ' - deoxyguanosine and DNA strand breakage , induced by reactive oxygen species due to metal ions in vitro , were observed using high performance liquid chromatograph coupled with electrochemical detection and single cell gel electrophoresis .

方法以正常人体外周血淋巴细胞为材料，采用单细胞凝胶电泳技术（SingleCellGelElectrophoresis，SCGE，又称彗星电泳），研究了不同剂量γ射线外照射诱导正常人血淋巴细胞DNA瞬时损伤的情况。

Methods The immediate irradiation-caused DNA damage in the human Peripheral Blood Lymphocytes irradiated with γ - rays was investigated . The single cell gel electrophoresis ( SCGE , Comet Assay ) were used to detect the extent of DNA damage .

采用放射免疫方法测定血清中IL2、IL6含量，以单细胞凝胶电泳技术测淋巴细胞DNA损伤程度。

The IL 2 and IL 6 levels in blood serum were measured by radioimmunoassay and lymphocyte DNA damage represented as comet tail length was determined by single cell gel electrophoresis ( SCGE ) .

胚胎植入期孕鼠暴露于不同剂量CS2以及不同时间点暴露于CS2，单细胞凝胶电泳技术（SCGE）检测脾脏淋巴细胞DNA损伤情况。

Mice were exposed CS2 at different dosages of CS2 or at different time-points during the phase of implantation in order to detect the DNA damage of lymphocytes in spleens by single cell gel electrophoresis ( SCGE ) . 3 .

方法体外培养的人肝细胞分别接触一定剂量的氟和（或）硒12h后，用单细胞凝胶电泳技术（SCGE）检测人肝细胞DNA的损伤率。

Methods The DNA damage rate in human hepatocytes was measured using single cell gel-electrophoresis assay after hepatocytes were incubated with fluoride ( 80 μ g / ml ) and / or selenium ( 1 . 73 μ g / ml ) for 12 h in vitro .

单细胞凝胶电泳技术用于生物体系辐射损伤评价

Application of Single Cell Gel Electrophoresis in Post-Evaluation of Organism Radiation Damage