免疫细胞化学技术

免疫细胞化学技术检测P－糖蛋白在人膀胱癌MDR细胞亚株中的表达

Detection of P-glycoprotein expression with immunocytochemistry in adriamycin-resistant human bladder cancer cell line

方法：采用免疫细胞化学技术分析患儿骨髓单个核细胞bcl-2、bax蛋白的表达。

Method : The expression of bcl 2 and bax protein in normal and leukemic mononuclear marrow cells were measured by immunocytochemistry staining .

方法应用Northern印迹杂交和免疫细胞化学技术。

Methods Northern blot analysis and immunocytochemical stain were used on cultured rat pulmonary fibroblasts ( PFBs ) after FN treatment .

应用免疫细胞化学技术检测用药前后凋亡相关基因bcl-2、bax表达的变化。

The expression of apoptosis associated genes bcl-2 and bax was observed by immunocytochemical staining .

方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA）的表达水平。

Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry .

方法通过形态学观察、MTT法检测、流式细胞技术分析、DNA琼脂糖凝胶电泳、免疫细胞化学技术研究Aβ、ROS对培养皮层神经细胞的损伤。

Methods The damage of A β and ROS on cultured cortical neurons was studied with morphological observation , MTT method assay , flow cytometric analysis , DNA agarose gel electrophoresis and immunocytochemistry staining method .

本文应用免疫细胞化学技术对新研究成功的结肠癌相关抗原LEA在光镜、电镜下进行了较详细的定位观察研究。

The distribution of LEA , a new colorectal carcinoma-associated antigen , was examined in details by immuno - light and immunoelectron microscopy .

采用免疫细胞化学技术对禾谷镰刀菌（Fusariumgraminearum）在侵染小麦穗部过程中产生的脱氧镰刀菌烯醇毒素（deoxynivalenol，DON）进行了定位分析。

Fusarium toxin deoxynivalenol ( DON ) was localized in wheat spikes during infection process of Fusarium graminearum by means of immunocytochemical labeling technique .

方法：对冲击波暴露后不同时间组采用免疫细胞化学技术（ABC）结合图像分析和听性脑干反应测试技术进行观测。

To further study the mechanism of blast trauma causing deafness . Methods : After shock wave , different groups of guinea pigs were studied by ABC immunohistochemistry , image analysis techniques and auditory brainstem response .

试验ⅣCajal间质细胞在中华鳖肠道的分布差异和超微特征本文应用c-Kit免疫细胞化学技术和透射电镜研究了Cajal间质细胞（ICC）在中华鳖肠道中的分布和超微结构特征。

By using c-Kit immuocytochemistry and transmission electron microscopy , this paper investigated the distribution and ultrastructure of interstitial cells of Cajal ( ICCs ) in the intestinal tract of Pelodiscus sinensis .

方法：应用AgNOR定量诊断和CEA、CK及VIM免疫细胞化学技术对56例浆膜腔积液进行对比研究。

Methods : We used AgNOR quantitative diagnosis and immunocytochemical techniques , including carcinoembryonic antigen ( CEA ), cytokeratin ( CK ) and vimentin ( VIM ) to determine the cells in serous fluid from 56 patients .

方法：应用原位杂交和免疫细胞化学技术，分别对人膀胱癌耐药细胞株（BIU87/ADM）及敏感细胞株（BIU87）中Bcl2基因及其产物的表达进行研究。

Methods : The expression of Bcl 2 gene in cell lines BIU 87 / ADM and BIU 87 was detected with in situ hybridization and immunohistochemistry .

用免疫细胞化学技术对大鼠脊髓背角内Fos阳性神经元和SP受体阳性神经元的分布及相互间关系进行了研究。

The present study was to investigate the distribution and relationship of c fos oncogene expressing protein product ( Fos ) positive neurons and substance P receptor ( SPR ) positive ones within the spinal dorsal horn of the rat by means of the immunocytochemistry .

以7例18至34周龄死亡人胎冠状动脉作材料，用免疫细胞化学技术ABC法显示NPY样神经纤维，用显微分光光度计扫描测定冠状动脉各分支NPY样神经纤维的含量。

The relative contents of NPY-Like nerve fibres in coronary artery branches were analyzed by scaning with a microspectrophotometer . The coronary artery branches come from seven cases of hu - man fetuses ranging from 18 to 34 weeks were stained by immunocytochemical ( ABC ) technique .

实验采用振荡分离和差速贴壁等方法进一步分离OPCs，通过免疫细胞化学技术对分离后的OPCs进行分析鉴定。

After the primary culture in vitro , the OPCs were further dissociated by the shaking process and differential adhesion . Then , the separated OPCs were also identified and analyzed by the immunocytochemical technique . 3 .

免疫细胞化学技术在转录因子激活研究中的应用

Ap plication of immunocytochemistry in the study of transcription factor activation

乳腺肿瘤细胞图像分析与免疫细胞化学技术的研究

The study on breast tumor cells by image analysis and immunocytochemistry

免疫细胞化学技术检测不孕妇女血清抗精子抗体

The detection of serum antisperm antibody of infertile women by immunochemical assay

免疫细胞化学技术在胸腹水脱落细胞学诊断中的应用

Application of immunocytochemistry to cytological diagnosis in hydrothorax and ascites

方法：采用细胞培养法和免疫细胞化学技术。

Methods : Immuno-cytochemical and cell culture were used in the experiment ;

应用免疫细胞化学技术对培养细胞及其分化细胞进行鉴定。

Immunohistochemistry were used to identify the type of cells .

免疫细胞化学技术、克隆分析等方法评价其生理特性。

Immunocytochemical techniques and clonal analysis were used to assess cellular physiological characteristics .

方法以成纤维细胞为实验对象，应用定量免疫细胞化学技术及粘附式激光扫描细胞仪，检测三维及二维培养系统中成纤维细胞内的Ⅰ型前胶原蛋白。

Methods Immunocytochemistry and ACAS were used to estimate procollagen in fibroblasts from three and two dimensional culture separately .

方法利用细胞培养和免疫细胞化学技术检测α-平滑肌肌动蛋白在体外培养的牙龈成纤维细胞与牙周韧带细胞中的表达。

Methods Periodontal ligament cells and gingival fibroblasts were cultured . Immunocytochemistry was used to detect the expression of α - MSA .

结论：用免疫细胞化学技术检测细胞核蛋白时，最好选用甲醇作为固定剂。

Conclusions : The results indicate that examination of nucleoproteins in cells by immunocytochemistry using the fixative like formaldehyde needs detergent extraction .

应用胞内注射生物胞素（bicytin），结合免疫细胞化学技术，我们研究了这两类神经元的形态学和免疫细胞化学方面的特征。

Using in vivo intracellular injection technique , combined with the technique of immunocytochemistry , we have studied the morphological and immunocytochemical features of the F-ERF and I-ERF neurons .

方法采用姬氏染色法初步鉴定大劣按蚊血细胞类型，继而利用免疫细胞化学技术检测大劣按蚊血细胞内前酚氧化酶和丝氨酸蛋白酶。

Methods The types of hemocytes were identified preliminarily by Giemsa s staining , and then both prophenoloxidase ( PPO ) and serine protease ( SP ) were detected by immunocytochemical technique .

方法采用细胞培养技术、荧光免疫细胞化学技术以及共焦激光扫描显微镜技术对神经干细胞向神经元定向分化过程中溶酶体和微管蛋白的分布变化进行观察。

[ Methods ] Using the techniques of cell culture , fluorescent immunocytochemistry and CLSM , we observed the distribution changes of lysosome and tubulin in neuron-oriented differentiation process of neural stem cells .

用原位核酸末端杂交和免疫细胞化学技术，分别观察28例16～32周人胎肺组织不同发育阶段的增殖与凋亡的表达特征。

28 cases of human fetal lung tissues ( from 16-32 weeks ) were studied by use of in situ hybridization and immuocytochemistry technique to examine the expressive characteristics of cell apoptosis and proliferating cell nuclear antigen ( PCNA ) in bronchial epithelial cells during the developmental stages .

通过免疫细胞化学染色技术检测NF-κB胞核阳性的淋巴细胞。

The immunocytochemical staining technique was used to detect activation of NF - κ B in lymphocytes .